Overview
- External ontology
- mediadive.medium:J995 (source link unavailable) — M30
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=995
Composition
flowchart LR
CultureMech_003344["m30"]
ing0_Sea_water["Sea water"]
CultureMech_003344 --> ing0_Sea_water
ing1_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_003344 --> ing1_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing1_Tris_HCl_buffer -.-> CHEBI_9754
ing2_N_Acetyl_D_glucosamine["N-Acetyl-D-glucosamine"]
CultureMech_003344 --> ing2_N_Acetyl_D_glucosamine
CHEBI_506227["CHEBI:506227"]
ing2_N_Acetyl_D_glucosamine -.-> CHEBI_506227
ing3_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_003344 --> ing3_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing3_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing4_Ampicillin["Ampicillin"]
CultureMech_003344 --> ing4_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing4_Ampicillin -.-> CHEBI_28971
ing5_Biotin["Biotin"]
CultureMech_003344 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003344 --> ing6_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing6_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_003344 --> ing7_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing8_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003344 --> ing8_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing8_Calcium_pantothenate -.-> CHEBI_31345
ing9_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003344 --> ing9_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing9_p_Aminobenzoic_acid -.-> CHEBI_30753
ing10_Folic_acid["Folic acid"]
CultureMech_003344 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Riboflavin["Riboflavin"]
CultureMech_003344 --> ing11_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing11_Riboflavin -.-> CHEBI_17015
ing12_Nicotinamide["Nicotinamide"]
CultureMech_003344 --> ing12_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing12_Nicotinamide -.-> CHEBI_17154
ing13_Vitamin_B12["Vitamin B12"]
CultureMech_003344 --> ing13_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing13_Vitamin_B12 -.-> CHEBI_176843
ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003344 --> ing14_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003344 --> ing15_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003344 --> ing16_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
ing17__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_003344 --> ing17__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing17__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003344 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea water | 700 G_PER_L | ||
| 2 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
50 G_PER_L |
CHEBI:9754
tris
|
|
| 3 |
N-Acetyl-D-glucosamine
C8H15NO6 221.209 g/mol
|
37.037 G_PER_L |
CHEBI:506227
N-acetyl-D-glucosamine
|
|
| 4 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.185185 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 5 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
4 G_PER_L |
CHEBI:28971
ampicillin
|
|
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004 G_PER_L |
CHEBI:15956
biotin
|
|
| 7 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.02 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 8 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 9 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 10 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.004 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 13 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.01 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 14 | Vitamin B12 | 0.0002 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 15 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 16 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 17 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.335 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 18 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.00925 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.099 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Preparation
- For solid medium, add 5.0 g/L gelrite (Gellan Gum).
- Add 0.2 ml filter-sterilized Solution 2 to Solution 1.
- Filter-sterilize.
- Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m30 (CultureMech:003344)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.258692Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J995 |
| 2026-03-13T06:19:19.680784Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003344 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.720012+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M30" to "m30" for consistent matching |
| 2026-06-05T07:41:11.834922+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.696856+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.920083+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 354deb2e2a8f461e4f58a1955dc9a83a1803722d024c66ed3c1a1d2058189046 |