m30

CultureMech:003344 bacterial COMPLEX LIQUID
Original name: M30

Overview

External ontology
mediadive.medium:J995 (source link unavailable) — M30
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=995

Composition

flowchart LR
    CultureMech_003344["m30"]
    ing0_Sea_water["Sea water"]
    CultureMech_003344 --> ing0_Sea_water
    ing1_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_003344 --> ing1_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing1_Tris_HCl_buffer -.-> CHEBI_9754
    ing2_N_Acetyl_D_glucosamine["N-Acetyl-D-glucosamine"]
    CultureMech_003344 --> ing2_N_Acetyl_D_glucosamine
    CHEBI_506227["CHEBI:506227"]
    ing2_N_Acetyl_D_glucosamine -.-> CHEBI_506227
    ing3_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_003344 --> ing3_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing3_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing4_Ampicillin["Ampicillin"]
    CultureMech_003344 --> ing4_Ampicillin
    CHEBI_28971["CHEBI:28971"]
    ing4_Ampicillin -.-> CHEBI_28971
    ing5_Biotin["Biotin"]
    CultureMech_003344 --> ing5_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing5_Biotin -.-> CHEBI_15956
    ing6_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003344 --> ing6_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing6_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_003344 --> ing7_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing8_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003344 --> ing8_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing8_Calcium_pantothenate -.-> CHEBI_31345
    ing9_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003344 --> ing9_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing9_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing10_Folic_acid["Folic acid"]
    CultureMech_003344 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Riboflavin["Riboflavin"]
    CultureMech_003344 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Nicotinamide["Nicotinamide"]
    CultureMech_003344 --> ing12_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing12_Nicotinamide -.-> CHEBI_17154
    ing13_Vitamin_B12["Vitamin B12"]
    CultureMech_003344 --> ing13_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing13_Vitamin_B12 -.-> CHEBI_176843
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003344 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003344 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003344 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_003344 --> ing17__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing17__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003344 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Sea water 700 G_PER_L
2 Tris-HCl buffer
C4H11NO3 121.136 g/mol
50 G_PER_L CHEBI:9754
tris
3 N-Acetyl-D-glucosamine
C8H15NO6 221.209 g/mol
37.037 G_PER_L CHEBI:506227
N-acetyl-D-glucosamine
4 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
0.185185 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
5 Ampicillin
C16H19N3O4S 349.412 g/mol
4 G_PER_L CHEBI:28971
ampicillin
6 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
7 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
8 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
9 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
10 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
11 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
12 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
13 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
14 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
18 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.099 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate

Preparation

  1. For solid medium, add 5.0 g/L gelrite (Gellan Gum).
  2. Add 0.2 ml filter-sterilized Solution 2 to Solution 1.
  3. Filter-sterilize.
  4. Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.258692Z mediadive-import Imported from MediaDive Source: JCM, ID: J995
2026-03-13T06:19:19.680784Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003344
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.720012+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M30" to "m30" for consistent matching
2026-06-05T07:41:11.834922+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.696856+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.