Overview
- External ontology
- komodo.medium:1116 (source link unavailable) — STEROIDOBACTER MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'steroidobacter_medium_replace_testosterone_with_heptanoate', 'source': 'KOMODO', 'source_id': 'komodo.medium:1116_replace_testosterone_with_heptanoate', 'original_category': 'bacterial'}
- pH
- 7.2
- Notes
- Source: KOMODO ModelSEED | ID: 1116 | DSMZ Medium: 1116 (mediadive.medium:1116) | Aerobic: Yes
Composition
flowchart LR
CultureMech_003818["steroidobacter_medium"]
ing0_NaCl["NaCl"]
CultureMech_003818 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003818 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_003818 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_KCl["KCl"]
CultureMech_003818 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NH4Cl["NH4Cl"]
CultureMech_003818 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003818 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Na2SO4["Na2SO4"]
CultureMech_003818 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_NaNO3["NaNO3"]
CultureMech_003818 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_HCl["HCl"]
CultureMech_003818 --> ing8_HCl
CHEBI_17883["CHEBI:17883"]
ing8_HCl -.-> CHEBI_17883
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003818 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_ZnCl2["ZnCl2"]
CultureMech_003818 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003818 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_003818 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003818 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003818 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003818 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003818 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_NaOH["NaOH"]
CultureMech_003818 --> ing17_NaOH
CHEBI_32145["CHEBI:32145"]
ing17_NaOH -.-> CHEBI_32145
ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003818 --> ing18_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003818 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_003818 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_Pantothenic_acid["Pantothenic acid"]
CultureMech_003818 --> ing21_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing21_Pantothenic_acid -.-> CHEBI_7916
ing22_Riboflavin["Riboflavin"]
CultureMech_003818 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_003818 --> ing23_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing23_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing24_Biotin["Biotin"]
CultureMech_003818 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_003818 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_003818 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_Nicotine_amide["Nicotine amide"]
CultureMech_003818 --> ing27_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing27_Nicotine_amide -.-> CHEBI_17154
ing28_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_003818 --> ing28_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_alpha_lipoic_acid -.-> CHEBI_16494
ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003818 --> ing29_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
CultureMech_003818 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.07 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
0.42 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 9 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 19 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 21 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 24 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 28 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 29 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 30 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 31 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_1116_STEROIDOBACTER_MEDIUM (CultureMech:003818)
- steroidobacter_medium (CultureMech:000552)
- steroidobacter_medium_replace_testosterone_with_heptanoate (CultureMech:003817)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1116 |
| 2026-02-03T11:18:57.114256 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1116 |
| 2026-03-13T06:19:22.493371Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003818 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:36.889059+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "STEROIDOBACTER MEDIUM" to "steroidobacter_medium" for consistent matching |
| 2026-06-05T07:40:19.105706+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.529816+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:40.181652+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_1116_STEROIDOBACTER_MEDIUM, steroidobacter_medium, steroidobacter_medium_replace_testosterone_with_heptanoate |
| 2026-08-06T03:04:40.181721+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint de686c1217a6b90f859c4273f022dec08813f8dd0a1eb1237687637028e9aea8 |