steroidobacter_medium

CultureMech:000552 bacterial DEFINED LIQUID
Original name: STEROIDOBACTER MEDIUM

Overview

External ontology
mediadive.medium:1116 (source link unavailable) — STEROIDOBACTER MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1116.pdf

Composition

flowchart LR
    CultureMech_000552["steroidobacter_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000552 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000552 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000552 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KCl["KCl"]
    CultureMech_000552 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_000552 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000552 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Na2SO4["Na2SO4"]
    CultureMech_000552 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_NaNO3["NaNO3"]
    CultureMech_000552 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_HCl["HCl"]
    CultureMech_000552 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000552 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_000552 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000552 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_000552 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000552 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000552 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000552 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000552 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NaOH["NaOH"]
    CultureMech_000552 --> ing17_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing17_NaOH -.-> CHEBI_32145
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000552 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000552 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_000552 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_Pantothenic_acid["Pantothenic acid"]
    CultureMech_000552 --> ing21_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing21_Pantothenic_acid -.-> CHEBI_7916
    ing22_Riboflavin["Riboflavin"]
    CultureMech_000552 --> ing22_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing22_Riboflavin -.-> CHEBI_17015
    ing23_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_000552 --> ing23_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing23_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing24_Biotin["Biotin"]
    CultureMech_000552 --> ing24_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_Biotin -.-> CHEBI_15956
    ing25_Folic_acid["Folic acid"]
    CultureMech_000552 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000552 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Nicotine_amide["Nicotine amide"]
    CultureMech_000552 --> ing27_Nicotine_amide
    CHEBI_17154["CHEBI:17154"]
    ing27_Nicotine_amide -.-> CHEBI_17154
    ing28_alpha_lipoic_acid["alpha-lipoic acid"]
    CultureMech_000552 --> ing28_alpha_lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_alpha_lipoic_acid -.-> CHEBI_16494
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000552 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_000552 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Na2SO4
2Na.O4S 142.043 g/mol
0.07 G_PER_L CHEBI:32149
sodium sulfate
8 NaNO3
NO3.Na 84.994 g/mol
0.42 G_PER_L CHEBI:63005
sodium nitrate
9 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
19 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
20 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
21 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
22 Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
23 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
24 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.01 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
25 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
26 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
27 Nicotinic acid
C6H5NO2 123.111 g/mol
0.025 G_PER_L CHEBI:15940
nicotinic acid
28 Nicotine amide
C6H6N2O 122.127 g/mol
0.025 G_PER_L CHEBI:17154
nicotinamide
29 alpha-lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
30 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
31 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.05 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. For growth with testosterone, dissolve testosterone in acetone (20 mg/ml), dispense portions of the solution, e.g. 0.1 ml portions for 10 ml medium, to anaerobic culture tubes. Let the solvent evaporate to dryness. Dispense 10 ml portions of medium and stream with N2/CO2 (80:20). Close the vessels and autoclave. Treat the vessels in an ultrasonic bath to detach and suspend the testosterone.
  2. For growth with heptanoate, omit testosterone. Sterilize the gassed medium in anaerobic vessels by autoclaving. After cooling, add 325 mg/l heptanoic acid from a 20fold, pH adjusted, filter sterilized anaerobic stock solution.
  3. After autoclaving, add (per 10 ml) 0.3 ml NaHCO3-solution (84 g/l, autoclaved under CO2-atmosphere), 100 µl trace element solution SL 10 (see below), 100 µl selenite tungstate solution (see below), and 100 µl vitamin solution (see below). Adjust pH to 7.2. Incubate the cultures in the dark for up to 4 weeks. Briefly shake the cultures once per day.
  4. Stir for some hours, filter sterilize the solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.430923Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1116
2026-03-13T06:18:56.336214Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000552
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.405660+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "STEROIDOBACTER MEDIUM" to "steroidobacter_medium" for consistent matching
2026-06-05T07:41:47.014600+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.841971+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.