Overview
- External ontology
- komodo.medium:1165 (source link unavailable) — SPIROCHAETA AMERICANA medium
- Applications
-
- Microbial cultivation
- pH
- 9.4
- Notes
- Source: KOMODO ModelSEED | ID: 1165 | DSMZ Medium: 1165 (mediadive.medium:1165) | Aerobic: No
Composition
flowchart LR
CultureMech_003878["spirochaeta_americana_medium"]
ing0_NaCl["NaCl"]
CultureMech_003878 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Na2CO3["Na2CO3"]
CultureMech_003878 --> ing1_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing1_Na2CO3 -.-> CHEBI_29377
ing2_NaHCO3["NaHCO3"]
CultureMech_003878 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_KCl["KCl"]
CultureMech_003878 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_K2HPO4["K2HPO4"]
CultureMech_003878 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003878 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_NH4Cl["NH4Cl"]
CultureMech_003878 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003878 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_Yeast_extract["Yeast extract"]
CultureMech_003878 --> ing8_Yeast_extract
ing9_D_Glucose["D-Glucose"]
CultureMech_003878 --> ing9_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing9_D_Glucose -.-> CHEBI_17634
ing10_Resazurin["Resazurin"]
CultureMech_003878 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Biotin["Biotin"]
CultureMech_003878 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_003878 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003878 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_003878 --> ing14_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing14_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing15_Riboflavin["Riboflavin"]
CultureMech_003878 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_003878 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_003878 --> ing17_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_D_Calcium_pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_003878 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003878 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_Lipoic_acid["Lipoic acid"]
CultureMech_003878 --> ing20_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20_Lipoic_acid -.-> CHEBI_16494
ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003878 --> ing21_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
ing22_Fe_NH4_2_SO4_2_x_12_H2O["Fe(NH4)2(SO4)2 x 12 H2O"]
CultureMech_003878 --> ing22_Fe_NH4_2_SO4_2_x_12_H2O
ing23_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003878 --> ing23_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing23_FeSO4_x_7_H2O -.-> CHEBI_75836
ing24_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003878 --> ing24_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing24_CoCl2_x_6_H2O -.-> CHEBI_53503
ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003878 --> ing25_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003878 --> ing26_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing27_NiCl2["NiCl2"]
CultureMech_003878 --> ing27_NiCl2
CHEBI_34887["CHEBI:34887"]
ing27_NiCl2 -.-> CHEBI_34887
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003878 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_003878 --> ing29_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing29_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
CultureMech_003878 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.76 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
24 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.4 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 9 | Yeast extract | 0.5 G_PER_L | ||
| 10 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
3.6 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 23 | Fe(NH4)2(SO4)2 x 12 H2O | 2 G_PER_L | ||
| 24 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 25 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 26 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 27 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 28 |
NiCl2
Cl2Ni 129.599 g/mol
|
0.5 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.1 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 31 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 32 |
HCl
HCl 36.461 g/mol
|
5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_1165_SPIROCHAETA_AMERICANA_medium (CultureMech:003878)
- spirochaeta_americana_medium (CultureMech:000606)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1165 |
| 2026-02-03T11:19:12.430660 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1165 |
| 2026-03-13T06:19:22.921050Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003878 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:37.383693+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIROCHAETA AMERICANA medium" to "spirochaeta_americana_medium" for consistent matching |
| 2026-06-05T07:40:19.502495+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.663785+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:40.817736+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_1165_SPIROCHAETA_AMERICANA_medium, spirochaeta_americana_medium |
| 2026-08-06T03:04:40.817779+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint a1ce36efaf4f9b60f4e7ac2c69436efffe25091fcacd64159f724cf94a52826a |