Overview
- External ontology
- mediadive.medium:1165 (source link unavailable) — SPIROCHAETA AMERICANA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1165.pdf
Composition
flowchart LR
CultureMech_000606["spirochaeta_americana_medium"]
ing0_NaCl["NaCl"]
CultureMech_000606 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Na2CO3["Na2CO3"]
CultureMech_000606 --> ing1_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing1_Na2CO3 -.-> CHEBI_29377
ing2_NaHCO3["NaHCO3"]
CultureMech_000606 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_KCl["KCl"]
CultureMech_000606 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_K2HPO4["K2HPO4"]
CultureMech_000606 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000606 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_NH4Cl["NH4Cl"]
CultureMech_000606 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000606 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_Yeast_extract["Yeast extract"]
CultureMech_000606 --> ing8_Yeast_extract
ing9_D_Glucose["D-Glucose"]
CultureMech_000606 --> ing9_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing9_D_Glucose -.-> CHEBI_17634
ing10_Resazurin["Resazurin"]
CultureMech_000606 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Biotin["Biotin"]
CultureMech_000606 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_000606 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000606 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000606 --> ing14_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing14_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing15_Riboflavin["Riboflavin"]
CultureMech_000606 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_000606 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_000606 --> ing17_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_D_Calcium_pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_000606 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000606 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_Lipoic_acid["Lipoic acid"]
CultureMech_000606 --> ing20_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20_Lipoic_acid -.-> CHEBI_16494
ing21_Fe_NH4_2_SO4_2_x_12_H2O["Fe(NH4)2(SO4)2 x 12 H2O"]
CultureMech_000606 --> ing21_Fe_NH4_2_SO4_2_x_12_H2O
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000606 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_NiCl2["NiCl2"]
CultureMech_000606 --> ing23_NiCl2
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2 -.-> CHEBI_34887
ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000606 --> ing24_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
ing25_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000606 --> ing25_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing25_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing26_H3BO3["H3BO3"]
CultureMech_000606 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_HCl["HCl"]
CultureMech_000606 --> ing27_HCl
CHEBI_17883["CHEBI:17883"]
ing27_HCl -.-> CHEBI_17883
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.76 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
24 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.4 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 9 | Yeast extract | 0.5 G_PER_L | ||
| 10 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 | Fe(NH4)2(SO4)2 x 12 H2O | 2 G_PER_L | ||
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
NiCl2
Cl2Ni 129.599 g/mol
|
0.5 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 26 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.1 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
HCl
HCl 36.461 g/mol
|
5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Preparation
- Adjust to pH 9.4
- Prepare the medium without the Na2S, yeast extract, glucose, Na2CO3, NaHCO3 and vitamins. Heat the medium in a boiling water bath and cool under a stream of nitrogen. Add the Na2CO3, and NaHCO3 to the cooled medium and check the pH. Dispense into appropriate vessels (Hungate tubes or serum bottles) and autoclave. To the sterile medium add Na2S, yeast extract, and glucose from autoclaved stock solutions. The vitamin solution is filter sterilised and added to the sterilised medium.
Solutions
Trace element solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MnCl2 x 4 H2O | 3.6 G_PER_L | |
| FeSO4 x 7 H2O | 1 G_PER_L | |
| CoCl2 x 6 H2O | 1 G_PER_L | |
| ZnSO4 x 7 H2O | 1 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.561311Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1165 |
| 2026-01-28T19:48:14.156438Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:56.754310Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000606 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.861803+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIROCHAETA AMERICANA MEDIUM" to "spirochaeta_americana_medium" for consistent matching |
| 2026-06-05T07:41:46.236444+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.599958+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:22.915412+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |