modified_medium_514

CultureMech:003893 bacterial COMPLEX LIQUID
Original name: MODIFIED medium 514

Overview

External ontology
komodo.medium:1173 (source link unavailable) — MODIFIED medium 514
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'marine_thermococcus_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:760', 'original_category': 'archaea'}
  • {'name': 'dsm_2476_dsm_5473_dsm_5474_dsm_9503_dsm_10343_dsm_10394_dsm_10395_dsm_11113_dsm_11836_dsm_12593_dsm_12819_dsm_12820_dsm_12597_dsm_12767_dsm_14981_dsm_15226_dsm_16538_dsm_17994_dsm_17882', 'source': 'KOMODO', 'source_id': 'komodo.medium:760.1', 'original_category': 'bacterial'}
  • {'name': 'bacto_marine_broth_difco_2216', 'source': 'MediaDive', 'source_id': 'mediadive.medium:514', 'original_category': 'specialized'}
  • {'name': 'marine_thermococcus_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:760', 'original_category': 'specialized'}
  • {'name': 'modified_bacto_marine_broth_difco_2216', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1517', 'original_category': 'specialized'}
Notes
Source: KOMODO ModelSEED | ID: 1173 | DSMZ Medium: 1173 (mediadive.medium:1173) | Aerobic: No

Composition

flowchart LR
    CultureMech_003893["modified_medium_514"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_003893 --> ing0_Bacto_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003893 --> ing1_Yeast_extract
    ing2_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_003893 --> ing2_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing2_Fe_III__citrate -.-> CHEBI_144421
    ing3_NaCl["NaCl"]
    CultureMech_003893 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2["MgCl2"]
    CultureMech_003893 --> ing4_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing4_MgCl2 -.-> CHEBI_6636
    ing5_Na2SO4["Na2SO4"]
    CultureMech_003893 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_CaCl2["CaCl2"]
    CultureMech_003893 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_KCl["KCl"]
    CultureMech_003893 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NaHCO3["NaHCO3"]
    CultureMech_003893 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_KBr["KBr"]
    CultureMech_003893 --> ing9_KBr
    CHEBI_32030["CHEBI:32030"]
    ing9_KBr -.-> CHEBI_32030
    ing10_SrCl2["SrCl2"]
    CultureMech_003893 --> ing10_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing10_SrCl2 -.-> CHEBI_36383
    ing11_H3BO3["H3BO3"]
    CultureMech_003893 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_Na_silicate["Na-silicate"]
    CultureMech_003893 --> ing12_Na_silicate
    CHEBI_60720["CHEBI:60720"]
    ing12_Na_silicate -.-> CHEBI_60720
    ing13_NaF["NaF"]
    CultureMech_003893 --> ing13_NaF
    CHEBI_28741["CHEBI:28741"]
    ing13_NaF -.-> CHEBI_28741
    ing14__NH4_NO3["(NH4)NO3"]
    CultureMech_003893 --> ing14__NH4_NO3
    CHEBI_63038["CHEBI:63038"]
    ing14__NH4_NO3 -.-> CHEBI_63038
    ing15_Na2HPO4["Na2HPO4"]
    CultureMech_003893 --> ing15_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing15_Na2HPO4 -.-> CHEBI_34683

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 5 G_PER_L
2 Yeast extract 1 G_PER_L
3 Fe(III) citrate
C6H5FeO7 244.944 g/mol
0.1 G_PER_L CHEBI:144421
iron(III) citrate
4 NaCl
Cl.Na 58.443 g/mol
19.45 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2
2Cl.Mg 95.211 g/mol
5.9 G_PER_L CHEBI:6636
magnesium dichloride
6 Na2SO4
2Na.O4S 142.043 g/mol
3.24 G_PER_L CHEBI:32149
sodium sulfate
7 CaCl2
Ca.2Cl 110.984 g/mol
1.8 G_PER_L CHEBI:3312
calcium dichloride
8 KCl
Cl.K 74.551 g/mol
0.55 G_PER_L CHEBI:32588
potassium chloride
9 NaHCO3
CHO3.Na 84.006 g/mol
0.16 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 KBr
Br.K 119.002 g/mol
0.08 G_PER_L CHEBI:32030
potassium bromide
11 SrCl2
2Cl.Sr 158.526 g/mol
0.034 G_PER_L CHEBI:36383
strontium dichloride
12 H3BO3
H3BO3 61.833 g/mol
0.022 G_PER_L CHEBI:33118
boric acid
13 Na-silicate
2Na.O3Si 122.063 g/mol
0.004 G_PER_L CHEBI:60720
sodium silicate
14 NaF
F.Na 41.988 g/mol
0.0024 G_PER_L CHEBI:28741
sodium fluoride
15 (NH4)NO3
H4N.NO3 80.043 g/mol
0.0016 G_PER_L CHEBI:63038
ammonium nitrate
16 Na2HPO4
HO4P.2Na 141.958 g/mol
0.008 G_PER_L CHEBI:34683
disodium hydrogenphosphate

Target organisms

No target organisms are recorded.

Provenance

Merged from 7 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:01.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 1173
2026-02-03T11:19:08.328779 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 1173
2026-03-13T06:19:23.061063Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003893
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:37.550790+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED medium 514" to "modified_medium_514" for consistent matching
2026-06-05T07:40:19.559802+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.682448+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:29.270119+00:00 recipe-merger Merged 7 duplicate recipes into canonical record Sources: marine_thermococcus_medium, KOMODO_1173_MODIFIED_medium_514, dsm_2476_dsm_5473_dsm_5474_dsm_9503_dsm_10343_dsm_10394_dsm_10395_dsm_11113_dsm_11836_dsm_12593_dsm_12819_dsm_12820_dsm_12597_dsm_12767_dsm_14981_dsm_15226_dsm_16538_dsm_17994_dsm_17882, modified_medium_514, bacto_marine_broth_difco_2216, marine_thermococcus_medium, modified_bacto_marine_broth_difco_2216
2026-08-06T03:04:29.270208+00:00 merge_recipes.py MERGED_RECIPES merged 7 source recipe(s) on fingerprint d0a8f83567ee2fa8e8c82657cfe7fb04f13bf4b773dc0d7b820c4508f3dd0944