Overview
- External ontology
- komodo.medium:1270 (source link unavailable) — B12 - MEDIUM GUTTMAN
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 1270 | DSMZ Medium: 1270 (mediadive.medium:1270) | Aerobic: No
Composition
flowchart LR
CultureMech_004025["b12_medium_guttman"]
ing0_KH2PO4["KH2PO4"]
CultureMech_004025 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_004025 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_004025 --> ing2_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing2_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004025 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_DL_Asparagine_x_H2O["DL-Asparagine x H2O"]
CultureMech_004025 --> ing4_DL_Asparagine_x_H2O
CHEBI_22653["CHEBI:22653"]
ing4_DL_Asparagine_x_H2O -.-> CHEBI_22653
ing5_L_Arginine_x_HCl["L-Arginine x HCl"]
CultureMech_004025 --> ing5_L_Arginine_x_HCl
CHEBI_31235["CHEBI:31235"]
ing5_L_Arginine_x_HCl -.-> CHEBI_31235
ing6_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_004025 --> ing6_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing6_L_Glutamic_acid -.-> CHEBI_16015
ing7_Glycine["Glycine"]
CultureMech_004025 --> ing7_Glycine
CHEBI_15428["CHEBI:15428"]
ing7_Glycine -.-> CHEBI_15428
ing8_L_Histidine["L-Histidine"]
CultureMech_004025 --> ing8_L_Histidine
CHEBI_15971["CHEBI:15971"]
ing8_L_Histidine -.-> CHEBI_15971
ing9_L_Tryptophan["L-Tryptophan"]
CultureMech_004025 --> ing9_L_Tryptophan
CHEBI_16828["CHEBI:16828"]
ing9_L_Tryptophan -.-> CHEBI_16828
ing10_L_Proline["L-Proline"]
CultureMech_004025 --> ing10_L_Proline
CHEBI_17203["CHEBI:17203"]
ing10_L_Proline -.-> CHEBI_17203
ing11__NH4_2SO4["(NH4)2SO4"]
CultureMech_004025 --> ing11__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing11__NH4_2SO4 -.-> CHEBI_62946
ing12_Glucose["Glucose"]
CultureMech_004025 --> ing12_Glucose
CHEBI_17234["CHEBI:17234"]
ing12_Glucose -.-> CHEBI_17234
ing13_Vitamin_B12["Vitamin B12"]
CultureMech_004025 --> ing13_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing13_Vitamin_B12 -.-> CHEBI_176843
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
1 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
DL-Asparagine x H2O
C4H8N2O3 132.119 g/mol
|
0.8 G_PER_L |
CHEBI:22653
asparagine
|
|
| 6 |
L-Arginine x HCl
C6H14N4O2.HCl 210.665 g/mol
|
0.2 G_PER_L |
CHEBI:31235
Arginine hydrochloride
|
|
| 7 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
0.2 G_PER_L |
CHEBI:16015
L-glutamic acid
|
|
| 8 |
Glycine
C2H5NO2 75.067 g/mol
|
0.2 G_PER_L |
CHEBI:15428
glycine
|
|
| 9 |
L-Histidine
C6H9N3O2 155.157 g/mol
|
0.2 G_PER_L |
CHEBI:15971
L-histidine
|
|
| 10 |
L-Tryptophan
C11H12N2O2 204.229 g/mol
|
0.2 G_PER_L |
CHEBI:16828
L-tryptophan
|
|
| 11 |
L-Proline
C5H9NO2 115.132 g/mol
|
0.2 G_PER_L |
CHEBI:17203
L-proline
|
|
| 12 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 13 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 14 | Vitamin B12 | 4e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_1270_B12_-_MEDIUM_GUTTMAN (CultureMech:004025)
- b12_medium_guttman (CultureMech:000732)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1270 |
| 2026-02-03T11:19:19.514674 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1270 |
| 2026-03-13T06:19:24.235019Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004025 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:38.942976+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "B12 - MEDIUM GUTTMAN" to "b12_medium_guttman" for consistent matching |
| 2026-05-20T06:00:49.857032+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2} |
| 2026-06-05T07:40:20.525158+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.998433+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:42.630148+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_1270_B12_-_MEDIUM_GUTTMAN, b12_medium_guttman |
| 2026-08-06T03:04:42.630187+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint d9c033c28efcc7c72a974c68ed9e7bbcd9e4a61c0c556601c4d64e4f9e96bf8d |