b12_medium_guttman

CultureMech:000732 bacterial DEFINED LIQUID
Original name: B12 - MEDIUM GUTTMAN

Overview

External ontology
mediadive.medium:1270 (source link unavailable) — B12 - MEDIUM GUTTMAN
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1270.pdf

Composition

flowchart LR
    CultureMech_000732["b12_medium_guttman"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000732 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000732 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_000732 --> ing2_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing2_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000732 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_DL_Asparagine_x_H2O["DL-Asparagine x H2O"]
    CultureMech_000732 --> ing4_DL_Asparagine_x_H2O
    CHEBI_22653["CHEBI:22653"]
    ing4_DL_Asparagine_x_H2O -.-> CHEBI_22653
    ing5_L_Arginine_x_HCl["L-Arginine x HCl"]
    CultureMech_000732 --> ing5_L_Arginine_x_HCl
    CHEBI_31235["CHEBI:31235"]
    ing5_L_Arginine_x_HCl -.-> CHEBI_31235
    ing6_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_000732 --> ing6_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing6_L_Glutamic_acid -.-> CHEBI_16015
    ing7_Glycine["Glycine"]
    CultureMech_000732 --> ing7_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing7_Glycine -.-> CHEBI_15428
    ing8_L_Histidine["L-Histidine"]
    CultureMech_000732 --> ing8_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing8_L_Histidine -.-> CHEBI_15971
    ing9_L_Tryptophan["L-Tryptophan"]
    CultureMech_000732 --> ing9_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing9_L_Tryptophan -.-> CHEBI_16828
    ing10_L_Proline["L-Proline"]
    CultureMech_000732 --> ing10_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing10_L_Proline -.-> CHEBI_17203
    ing11__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000732 --> ing11__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing11__NH4_2SO4 -.-> CHEBI_62946
    ing12_Glucose["Glucose"]
    CultureMech_000732 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_Vitamin_B12["Vitamin B12"]
    CultureMech_000732 --> ing13_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing13_Vitamin_B12 -.-> CHEBI_176843

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
14 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
1 G_PER_L CHEBI:53258
sodium citrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 DL-Asparagine x H2O
C4H8N2O3 132.119 g/mol
0.8 G_PER_L CHEBI:22653
asparagine
6 L-Arginine x HCl
C6H14N4O2.HCl 210.665 g/mol
0.2 G_PER_L CHEBI:31235
Arginine hydrochloride
7 L-Glutamic acid
C5H9NO4 147.13 g/mol
0.2 G_PER_L CHEBI:16015
L-glutamic acid
8 Glycine
C2H5NO2 75.067 g/mol
0.2 G_PER_L CHEBI:15428
glycine
9 L-Histidine
C6H9N3O2 155.157 g/mol
0.2 G_PER_L CHEBI:15971
L-histidine
10 L-Tryptophan
C11H12N2O2 204.229 g/mol
0.2 G_PER_L CHEBI:16828
L-tryptophan
11 L-Proline
C5H9NO2 115.132 g/mol
0.2 G_PER_L CHEBI:17203
L-proline
12 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
13 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
14 Vitamin B12 4e-05 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Adjust pH to 6.8 - 7.2.
  2. Final concentration of vitamin B12: 40 µg/L.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.926286Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1270
2026-01-28T19:48:27.783875Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:57.876194Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000732
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.206925+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "B12 - MEDIUM GUTTMAN" to "b12_medium_guttman" for consistent matching
2026-05-20T06:01:09.391474+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2}
2026-06-05T07:40:50.186828+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.685050+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.