Overview
- External ontology
- komodo.medium:205 (source link unavailable) — NATRONOBACTERIUM medium
- Applications
-
- Microbial cultivation
- pH
- 8.5
- High-metal flag
- True
- Notes
- pH buffer: Na2CO3 | Source: KOMODO ModelSEED | ID: 205 | DSMZ Medium: 205 (mediadive.medium:205) | Aerobic: No
Composition
flowchart LR
CultureMech_004333["natronobacterium_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_004333 --> ing0_Casamino_acids
ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_004333 --> ing1_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing2_Glutamic_acid["Glutamic acid"]
CultureMech_004333 --> ing2_Glutamic_acid
CHEBI_18237["CHEBI:18237"]
ing2_Glutamic_acid -.-> CHEBI_18237
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004333 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_KCl["KCl"]
CultureMech_004333 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaCl["NaCl"]
CultureMech_004333 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Agar["Agar"]
CultureMech_004333 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Na2CO3["Na2CO3"]
CultureMech_004333 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 15 G_PER_L | ||
| 2 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 |
Glutamic acid
C5H9NO4 147.13 g/mol
|
2.5 G_PER_L |
CHEBI:18237
glutamic acid
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
250 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
variable VARIABLE |
CHEBI:29377
sodium carbonate
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_205_NATRONOBACTERIUM_medium (CultureMech:004333)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 205 |
| 2026-02-03T11:19:11.235146 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 205 |
| 2026-03-13T03:09:22.174449Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: Na2CO3 |
| 2026-03-13T06:19:26.603091Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004333 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:41.719125+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NATRONOBACTERIUM medium" to "natronobacterium_medium" for consistent matching |
| 2026-05-16T09:03:01.687372+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:21.606851+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.814950+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:44.359187+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:22.728019+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3f250bae798ccdc911ba231fe93efb55853ef71cea6cbffac381848e2a43d306 |