natronobacterium_medium (CultureMech:004333)

Overview

External ontology

komodo.medium:205 (source link unavailable) — NATRONOBACTERIUM medium

Applications

Microbial cultivation

pH

8.5

High-metal flag

True

Notes

pH buffer: Na2CO3 | Source: KOMODO ModelSEED | ID: 205 | DSMZ Medium: 205 (mediadive.medium:205) | Aerobic: No

Composition

flowchart LR
    CultureMech_004333["natronobacterium_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_004333 --> ing0_Casamino_acids
    ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_004333 --> ing1_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing2_Glutamic_acid["Glutamic acid"]
    CultureMech_004333 --> ing2_Glutamic_acid
    CHEBI_18237["CHEBI:18237"]
    ing2_Glutamic_acid -.-> CHEBI_18237
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004333 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KCl["KCl"]
    CultureMech_004333 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_004333 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_004333 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Na2CO3["Na2CO3"]
    CultureMech_004333 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	Casamino acids	15 G_PER_L
2	Trisodium citrate x 2 H2O C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
3	Glutamic acid C5H9NO4 147.13 g/mol	2.5 G_PER_L	CHEBI:18237 glutamic acid
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	2.5 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
5	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride
6	NaCl Cl.Na 58.443 g/mol	250 G_PER_L	CHEBI:26710 sodium chloride
7	Agar	20 G_PER_L	CHEBI:2509 agar
8	Na2CO3 CO3.2Na 105.988 g/mol	variable VARIABLE	CHEBI:29377 sodium carbonate	pH adjustment (extracted from notes)

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 205
2026-02-03T11:19:11.235146	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 205
2026-03-13T03:09:22.174449Z	data-quality-cleanup-v1.0	Data quality fixes	Added pH buffer: Na2CO3
2026-03-13T06:19:26.603091Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004333
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Na2CO3
2026-03-17T06:30:30.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:41.719125+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "NATRONOBACTERIUM medium" to "natronobacterium_medium" for consistent matching
2026-05-16T09:03:01.687372+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:21.606851+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.814950+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:44.359187+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.