bacillus_schlegelii_heterotrophic_medium

CultureMech:004664 bacterial DEFINED SOLID_AGAR
Original name: BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM

Overview

External ontology
komodo.medium:260 (source link unavailable) — BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: KOMODO ModelSEED | ID: 260 | DSMZ Medium: 260 (mediadive.medium:260) | Aerobic: No

Composition

flowchart LR
    CultureMech_004664["bacillus_schlegelii_heterotrophic_medium"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_004664 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_004664 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_004664 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_004664 --> ing3_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing3_MnSO4_x_H2O -.-> CHEBI_86364
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004664 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004664 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_004664 --> ing6_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing6_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing7_Na_pyruvate["Na-pyruvate"]
    CultureMech_004664 --> ing7_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing7_Na_pyruvate -.-> CHEBI_50144
    ing8_Agar["Agar"]
    CultureMech_004664 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_004664 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_004664 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_004664 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004664 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_004664 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004664 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004664 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
4.5 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.01 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Ferric ammonium citrate 0.005 G_PER_L CHEBI:31604
ferric ammonium citrate
8 Na-pyruvate
C3H3O3.Na 110.044 g/mol
1.5 G_PER_L CHEBI:50144
sodium pyruvate
9 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 260
2026-02-03T11:18:58.499891 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 260
2026-03-13T06:19:27.946727Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004664
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:43.255666+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM" to "bacillus_schlegelii_heterotrophic_medium" for consistent matching
2026-06-05T07:40:21.943854+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:44.463262+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:46.278697+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: KOMODO_260_BACILLUS_SCHLEGELII_HETEROTROPHIC_MEDIUM, bacillus_schlegelii_heterotrophic_medium
2026-08-06T03:04:46.278742+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 7733280f38fd4ec7f923cd67245bd92a74a2cbfd80d35bb205d8c3a5b0109bbc