Overview
- External ontology
- mediadive.medium:260 (source link unavailable) — BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium260.pdf
Composition
flowchart LR
CultureMech_001359["bacillus_schlegelii_heterotrophic_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001359 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_001359 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_001359 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001359 --> ing3_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing3_MnSO4_x_H2O -.-> CHEBI_86364
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001359 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001359 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_001359 --> ing6_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing6_Ferric_ammonium_citrate -.-> CHEBI_31604
ing7_Na_pyruvate["Na-pyruvate"]
CultureMech_001359 --> ing7_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Na_pyruvate -.-> CHEBI_50144
ing8_Agar["Agar"]
CultureMech_001359 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Distilled_water["Distilled water"]
CultureMech_001359 --> ing9_Distilled_water
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
4.5 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.01 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 | Ferric ammonium citrate | 0.005 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 8 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
1.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 10 | Distilled water | 1 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
Preparation
- Adjust pH to 7.1. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121°C. Incubate without agitation at 65°C.
Solutions
Trace element solution SL-6
Dose: 0.003 L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnSO4 x 7 H2O | 0.1 G_PER_L | |
| MnCl2 x 4 H2O | 0.03 G_PER_L | |
| H3BO3 | 0.3 G_PER_L | |
| CoCl2 x 6 H2O | 0.2 G_PER_L | |
| CuCl2 x 2 H2O | 0.01 G_PER_L | |
| NiCl2 x 6 H2O | 0.02 G_PER_L | |
| Na2MoO4 x 2 H2O | 0.03 G_PER_L | |
| Distilled water | 1 L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.607821Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 260 |
| 2026-03-13T06:19:03.383227Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001359 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.304519+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM" to "bacillus_schlegelii_heterotrophic_medium" for consistent matching |
| 2026-06-05T07:40:50.320595+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.732465+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T11:35:03.558874+00:00 | codex | RESTORED_REVIEWED_MEDIUM_STOCK_BOUNDARIES | Exact source graph, complete ingredient inventory, amounts, and original PDF recipe chain checked. Numeric concentrations and chemical mappings preserved; stock doses and water are explicit batch amounts. No final-medium stock dilution inferred. Parent/variant relationships remain separately unreviewed. |