Overview
- External ontology
- komodo.medium:342 (source link unavailable) — METHANOBACTERIUM ALCALIPHILUM medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 8.3, 'max': 8.4}
- Notes
- Source: KOMODO ModelSEED | ID: 342 | DSMZ Medium: 342 (mediadive.medium:342) | Aerobic: Yes
Composition
flowchart LR
CultureMech_005050["methanobacterium_alcaliphilum_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_005050 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005050 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005050 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_K2HPO4["K2HPO4"]
CultureMech_005050 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_005050 --> ing4_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing4_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing5_Yeast_extract["Yeast extract"]
CultureMech_005050 --> ing5_Yeast_extract
ing6_Trypticase_peptone["Trypticase peptone"]
CultureMech_005050 --> ing6_Trypticase_peptone
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_005050 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_005050 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_NaHCO3["NaHCO3"]
CultureMech_005050 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Na2CO3["Na2CO3"]
CultureMech_005050 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_005050 --> ing11_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing11_Tris_HCl_buffer -.-> CHEBI_9754
ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_005050 --> ing12_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005050 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_005050 --> ing14_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing14_MnSO4_x_H2O -.-> CHEBI_86364
ing15_NaCl["NaCl"]
CultureMech_005050 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005050 --> ing16_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_005050 --> ing17_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_005050 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_005050 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_005050 --> ing20_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing21_H3BO3["H3BO3"]
CultureMech_005050 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005050 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005050 --> ing23_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_005050 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.26263 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.126263 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.226263 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.126263, 0.1] |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.50505 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.000552525 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
[Merged 2 duplicates: 0.000252525, 0.0003] |
| 6 | Yeast extract | 2.52525 G_PER_L | ||
| 7 | Trypticase peptone | 2.52525 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000631313 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.631313 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
47.619 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.38095 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
10 G_PER_L |
CHEBI:9754
tris
|
|
| 13 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 15 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 17 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 18 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 21 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_342_METHANOBACTERIUM_ALCALIPHILUM_medium (CultureMech:005050)
- methanobacterium_alcaliphilum_medium (CultureMech:001442)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 342 |
| 2026-02-03T11:18:54.848892 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 342 |
| 2026-03-13T03:08:39.152265Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:29.788722Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005050 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.415417+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOBACTERIUM ALCALIPHILUM medium" to "methanobacterium_alcaliphilum_medium" for consistent matching |
| 2026-05-20T06:00:51.311198+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '8.3-8.4' -> {'min': 8.3, 'max': 8.4} |
| 2026-06-05T07:40:22.592695+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.640408+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:22.972629+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_342_METHANOBACTERIUM_ALCALIPHILUM_medium, methanobacterium_alcaliphilum_medium |
| 2026-08-06T03:04:22.972691+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 7ef335bb30778d61cde26d3e0b8b485ce0e43d3721107679b58e1f45b0786d7c |