methanobacterium_alcaliphilum_medium

CultureMech:001442 archaea COMPLEX LIQUID
Original name: METHANOBACTERIUM ALCALIPHILUM MEDIUM

Overview

External ontology
mediadive.medium:342 (source link unavailable) — METHANOBACTERIUM ALCALIPHILUM MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 8.3, 'max': 8.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium342.pdf

Composition

flowchart LR
    CultureMech_001442["methanobacterium_alcaliphilum_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001442 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001442 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001442 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_K2HPO4["K2HPO4"]
    CultureMech_001442 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001442 --> ing4_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing4_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_001442 --> ing5_Yeast_extract
    ing6_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001442 --> ing6_Trypticase_peptone
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001442 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001442 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_NaHCO3["NaHCO3"]
    CultureMech_001442 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2CO3["Na2CO3"]
    CultureMech_001442 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_001442 --> ing11_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing11_Tris_HCl_buffer -.-> CHEBI_9754
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001442 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001442 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001442 --> ing14_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing14_MnSO4_x_H2O -.-> CHEBI_86364
    ing15_NaCl["NaCl"]
    CultureMech_001442 --> ing15_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing15_NaCl -.-> CHEBI_26710
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001442 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001442 --> ing17_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001442 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001442 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001442 --> ing20_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing21_H3BO3["H3BO3"]
    CultureMech_001442 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001442 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001442 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001442 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1.26263 G_PER_L CHEBI:31206
ammonium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.126263 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.226263 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.126263, 0.1]
4 K2HPO4
HO4P.2K 174.174 g/mol
0.50505 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.000552525 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 [Merged 2 duplicates: 0.000252525, 0.0003]
6 Yeast extract 2.52525 G_PER_L
7 Trypticase peptone 2.52525 G_PER_L
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000631313 G_PER_L CHEBI:8806
Resazurin
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.631313 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 NaHCO3
CHO3.Na 84.006 g/mol
47.619 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Na2CO3
CO3.2Na 105.988 g/mol
2.38095 G_PER_L CHEBI:29377
sodium carbonate
12 Tris-HCl buffer
C4H11NO3 121.136 g/mol
10 G_PER_L CHEBI:9754
tris
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
15 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
16 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
22 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
25 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients of solution A (except cysteine), bring to the boil, then cool to room temperature under 100% H2 gas. Add cysteine and adjust pH to 7.0, if necessary. Dispense medium under 100% H2 gas atmosphere into anoxic Hungate-type tubes or serum vials vials to 30% of their volume and then autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere. Complete the medium by adding appropriate amounts of solutions B and C to the sterile solution A. Adjust pH of complete medium to 8.3 - 8.4, if necessary.
  2. After inoculation add sterile H2 gas to 1 - 2 bar overpressure.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.855749Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 342
2026-03-13T03:08:39.082572Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:04.225387Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001442
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.224128+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOBACTERIUM ALCALIPHILUM MEDIUM" to "methanobacterium_alcaliphilum_medium" for consistent matching
2026-05-20T06:01:54.981804+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '8.3-8.4' -> {'min': 8.3, 'max': 8.4}
2026-06-05T07:41:30.619666+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.626881+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.