mineral_medium_m9_for_e_coli_jm_strains (CultureMech:005129)

Overview

External ontology

komodo.medium:382 (source link unavailable) — MINERAL MEDIUM M9 for E. coli JM strains

Applications

Microbial cultivation

pH

7.4

Notes

Source: KOMODO ModelSEED | ID: 382 | DSMZ Medium: 382 (mediadive.medium:382) | Aerobic: No

Composition

flowchart LR
    CultureMech_005129["mineral_medium_m9_for_e_coli_jm_strains"]
    ing0_MgSO4["MgSO4"]
    CultureMech_005129 --> ing0_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing0_MgSO4 -.-> CHEBI_32599
    ing1_CaCl2["CaCl2"]
    CultureMech_005129 --> ing1_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing1_CaCl2 -.-> CHEBI_3312
    ing2_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005129 --> ing2_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing2_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing3_Glucose["Glucose"]
    CultureMech_005129 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Na2HPO4["Na2HPO4"]
    CultureMech_005129 --> ing4_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4 -.-> CHEBI_34683
    ing5_KH2PO4["KH2PO4"]
    CultureMech_005129 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_005129 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_NaCl["NaCl"]
    CultureMech_005129 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710

Ingredients (8)

#	Ingredient	Concentration	Ontology
1	MgSO4 Mg.O4S 120.368 g/mol	0.12037 G_PER_L	CHEBI:32599 magnesium sulfate
2	CaCl2 Ca.2Cl 110.984 g/mol	0.011098 G_PER_L	CHEBI:3312 calcium dichloride
3	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.373301 G_PER_L	CHEBI:132751 thiamine hydrochloride dihydrate
4	Glucose C6H12O6 180.156 g/mol	2 G_PER_L	CHEBI:17234 glucose
5	Na2HPO4 HO4P.2Na 141.958 g/mol	60 G_PER_L	CHEBI:34683 disodium hydrogenphosphate
6	KH2PO4 H2O4P.K 136.084 g/mol	30 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
7	NH4Cl Cl.H4N 53.492 g/mol	10 G_PER_L	CHEBI:31206 ammonium chloride
8	NaCl Cl.Na 58.443 g/mol	5 G_PER_L	CHEBI:26710 sodium chloride

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

KOMODO_382_MINERAL_MEDIUM_M9_for_E._coli_JM_strains (CultureMech:005129)
mineral_medium_m9_for_e_coli_jm_strains (CultureMech:001488)

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 382
2026-02-03T11:19:08.882552	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 382
2026-03-13T06:19:30.284614Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005129
2026-03-17T06:30:23.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:46.007749+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MINERAL MEDIUM M9 for E. coli JM strains" to "mineral_medium_m9_for_e_coli_jm_strains" for consistent matching
2026-06-05T07:40:22.867737+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:44.733583+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:48.064792+00:00	recipe-merger	Merged 2 duplicate recipes into canonical record	Sources: KOMODO_382_MINERAL_MEDIUM_M9_for_E._coli_JM_strains, mineral_medium_m9_for_e_coli_jm_strains
2026-08-06T03:04:48.064847+00:00	merge_recipes.py	MERGED_RECIPES	merged 2 source recipe(s) on fingerprint 261a1ed68a65f416596cd6969234d8a02385e22517c39b8aed979fe5b6e3e59b