mineral_medium_m9_for_e_coli_jm_strains

CultureMech:001488 bacterial DEFINED LIQUID
Original name: MINERAL MEDIUM M9 for E. coli JM strains

Overview

External ontology
mediadive.medium:382 (source link unavailable) — MINERAL MEDIUM M9 for E. coli JM strains
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium382.pdf

Composition

flowchart LR
    CultureMech_001488["mineral_medium_m9_for_e_coli_jm_strains"]
    ing0_MgSO4["MgSO4"]
    CultureMech_001488 --> ing0_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing0_MgSO4 -.-> CHEBI_32599
    ing1_CaCl2["CaCl2"]
    CultureMech_001488 --> ing1_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing1_CaCl2 -.-> CHEBI_3312
    ing2_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001488 --> ing2_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing2_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing3_Glucose["Glucose"]
    CultureMech_001488 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Na2HPO4["Na2HPO4"]
    CultureMech_001488 --> ing4_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4 -.-> CHEBI_34683
    ing5_KH2PO4["KH2PO4"]
    CultureMech_001488 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_001488 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_NaCl["NaCl"]
    CultureMech_001488 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 MgSO4
Mg.O4S 120.368 g/mol
0.12037 G_PER_L CHEBI:32599
magnesium sulfate
2 CaCl2
Ca.2Cl 110.984 g/mol
0.011098 G_PER_L CHEBI:3312
calcium dichloride
3 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.373301 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
4 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
5 Na2HPO4
HO4P.2Na 141.958 g/mol
60 G_PER_L CHEBI:34683
disodium hydrogenphosphate
6 KH2PO4
H2O4P.K 136.084 g/mol
30 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 NH4Cl
Cl.H4N 53.492 g/mol
10 G_PER_L CHEBI:31206
ammonium chloride
8 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving.
  2. Adjust pH to 7.4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.976887Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 382
2026-03-13T06:19:04.582631Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001488
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.619199+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM M9 for E. coli JM strains" to "mineral_medium_m9_for_e_coli_jm_strains" for consistent matching
2026-06-05T07:41:34.185378+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.897586+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.