Overview
- External ontology
- komodo.medium:441 (source link unavailable) — DIAZOTROPHIC medium (RBA)
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: KOMODO ModelSEED | ID: 441 | DSMZ Medium: 441 (mediadive.medium:441) | Aerobic: No
Composition
flowchart LR
CultureMech_005288["diazotrophic_medium_rba"]
ing0_KH2PO4["KH2PO4"]
CultureMech_005288 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_005288 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NaCl["NaCl"]
CultureMech_005288 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005288 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005288 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005288 --> ing5_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing6_NaVO3_x_H2O["NaVO3 x H2O"]
CultureMech_005288 --> ing6_NaVO3_x_H2O
CHEBI_132095["CHEBI:132095"]
ing6_NaVO3_x_H2O -.-> CHEBI_132095
ing7_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_005288 --> ing7_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing7_MnSO4_x_H2O -.-> CHEBI_86364
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005288 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_Yeast_extract["Yeast extract"]
CultureMech_005288 --> ing9_Yeast_extract
ing10_Agar["Agar"]
CultureMech_005288 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_Disodium_succinate["Disodium succinate"]
CultureMech_005288 --> ing11_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing11_Disodium_succinate -.-> CHEBI_63675
ing12_DL_Malate["DL-Malate"]
CultureMech_005288 --> ing12_DL_Malate
CHEBI_15595["CHEBI:15595"]
ing12_DL_Malate -.-> CHEBI_15595
ing13_Na_pyruvate["Na-pyruvate"]
CultureMech_005288 --> ing13_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing13_Na_pyruvate -.-> CHEBI_50144
ing14_D_Mannitol["D-Mannitol"]
CultureMech_005288 --> ing14_D_Mannitol
CHEBI_16899["CHEBI:16899"]
ing14_D_Mannitol -.-> CHEBI_16899
ing15_D_Glucose["D-Glucose"]
CultureMech_005288 --> ing15_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing15_D_Glucose -.-> CHEBI_17634
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_005288 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005288 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_005288 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005288 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005288 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005288 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Riboflavin["Riboflavin"]
CultureMech_005288 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_005288 --> ing23_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing23_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_005288 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_005288 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_005288 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Biotin["Biotin"]
CultureMech_005288 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Folic_acid["Folic acid"]
CultureMech_005288 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_005288 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.104932 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.944386 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.104932 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.104932 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.104932 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03524659 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Merged 2 duplicates: 0.00524659, 0.03] |
| 7 |
NaVO3 x H2O
H2O.Na.O3V 139.946 g/mol
|
0.00524659 G_PER_L |
CHEBI:132095
sodium metavanadate hydrate
|
|
| 8 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.00524659 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0104932 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 | Yeast extract | 0.0524659 G_PER_L | ||
| 11 | Agar | 15.7398 G_PER_L |
CHEBI:2509
agar
|
(if required) |
| 12 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
20 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 13 |
DL-Malate
C4H4O5 132.071 g/mol
|
40 G_PER_L |
CHEBI:15595
malate(2-)
|
|
| 14 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
20 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 15 |
D-Mannitol
C6H14O6 182.172 g/mol
|
40 G_PER_L |
CHEBI:16899
D-mannitol
|
|
| 16 |
D-Glucose
C6H12O6 180.156 g/mol
|
40 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.1 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 24 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.5 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.5 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.5 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_441_DIAZOTROPHIC_medium_RBA (CultureMech:005288)
- diazotrophic_medium_rba (CultureMech:001550)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 441 |
| 2026-02-03T11:19:13.930045 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 441 |
| 2026-03-13T03:09:29.745290Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:31.529288Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005288 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:47.421329+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DIAZOTROPHIC medium (RBA)" to "diazotrophic_medium_rba" for consistent matching |
| 2026-06-05T07:40:23.364058+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.904068+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:49.427806+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_441_DIAZOTROPHIC_medium_RBA, diazotrophic_medium_rba |
| 2026-08-06T03:04:49.427852+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ea71abf4412aeaab37f2170975d7959aea4f46002509f1eff3974bd0421311f1 |