diazotrophic_medium_rba

CultureMech:005288 bacterial COMPLEX SOLID_AGAR
Original name: DIAZOTROPHIC medium (RBA)

Overview

External ontology
komodo.medium:441 (source link unavailable) — DIAZOTROPHIC medium (RBA)
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: KOMODO ModelSEED | ID: 441 | DSMZ Medium: 441 (mediadive.medium:441) | Aerobic: No

Composition

flowchart LR
    CultureMech_005288["diazotrophic_medium_rba"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_005288 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_005288 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NaCl["NaCl"]
    CultureMech_005288 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005288 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005288 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005288 --> ing5_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing5_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing6_NaVO3_x_H2O["NaVO3 x H2O"]
    CultureMech_005288 --> ing6_NaVO3_x_H2O
    CHEBI_132095["CHEBI:132095"]
    ing6_NaVO3_x_H2O -.-> CHEBI_132095
    ing7_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_005288 --> ing7_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing7_MnSO4_x_H2O -.-> CHEBI_86364
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005288 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_005288 --> ing9_Yeast_extract
    ing10_Agar["Agar"]
    CultureMech_005288 --> ing10_Agar
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar -.-> CHEBI_2509
    ing11_Disodium_succinate["Disodium succinate"]
    CultureMech_005288 --> ing11_Disodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing11_Disodium_succinate -.-> CHEBI_63675
    ing12_DL_Malate["DL-Malate"]
    CultureMech_005288 --> ing12_DL_Malate
    CHEBI_15595["CHEBI:15595"]
    ing12_DL_Malate -.-> CHEBI_15595
    ing13_Na_pyruvate["Na-pyruvate"]
    CultureMech_005288 --> ing13_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing13_Na_pyruvate -.-> CHEBI_50144
    ing14_D_Mannitol["D-Mannitol"]
    CultureMech_005288 --> ing14_D_Mannitol
    CHEBI_16899["CHEBI:16899"]
    ing14_D_Mannitol -.-> CHEBI_16899
    ing15_D_Glucose["D-Glucose"]
    CultureMech_005288 --> ing15_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing15_D_Glucose -.-> CHEBI_17634
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005288 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005288 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_005288 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005288 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005288 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005288 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_005288 --> ing22_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_Calcium_pantothenate -.-> CHEBI_31345
    ing23_Biotin["Biotin"]
    CultureMech_005288 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_005288 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Vitamin_B12["Vitamin B12"]
    CultureMech_005288 --> ing25_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing25_Vitamin_B12 -.-> CHEBI_176843

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.104932 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.944386 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
0.104932 G_PER_L CHEBI:26710
sodium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.104932 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.104932 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03524659 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 [Merged 2 duplicates: 0.00524659, 0.03]
7 NaVO3 x H2O
H2O.Na.O3V 139.946 g/mol
0.00524659 G_PER_L CHEBI:132095
sodium metavanadate hydrate
8 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.00524659 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0104932 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 Yeast extract 0.0524659 G_PER_L
11 Agar 15.7398 G_PER_L CHEBI:2509
agar
 (if required)
12 Disodium succinate
C4H4O4.2Na 162.052 g/mol
20 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
13 DL-Malate
C4H4O5 132.071 g/mol
40 G_PER_L CHEBI:15595
malate(2-)
14 Na-pyruvate
C3H3O3.Na 110.044 g/mol
20 G_PER_L CHEBI:50144
sodium pyruvate
15 D-Mannitol
C6H14O6 182.172 g/mol
40 G_PER_L CHEBI:16899
D-mannitol
16 D-Glucose
C6H12O6 180.156 g/mol
40 G_PER_L CHEBI:17634
D-glucose
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
23 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.5 G_PER_L CHEBI:31345
Calcium pantothenate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
25 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
26 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12

Solutions

Standard vitamin solution

Dose: 5 ML_PER_L

Stock composition
IngredientConcentrationNotes
Riboflavin 0.1 G_PER_L
Thiamine-HCl x 2 H2O 0.5 G_PER_L
Nicotinic acid 0.5 G_PER_L
Pyridoxine hydrochloride 0.5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 441
2026-02-03T11:19:13.930045 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 441
2026-03-13T03:09:29.745290Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:31.529288Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005288
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:47.421329+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DIAZOTROPHIC medium (RBA)" to "diazotrophic_medium_rba" for consistent matching
2026-06-05T07:40:23.364058+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:44.904068+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T20:44:33.455341+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Standard vitamin solution' @ 5 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).