Overview
- External ontology
- komodo.medium:452 (source link unavailable) — GLUCOSE medium (NAKAYAMA)
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: KOMODO ModelSEED | ID: 452 | DSMZ Medium: 452 (mediadive.medium:452) | Aerobic: No
Composition
flowchart LR
CultureMech_005291["glucose_medium_nakayama"]
ing0_Glucose["Glucose"]
CultureMech_005291 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Peptone["Peptone"]
CultureMech_005291 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_005291 --> ing2_Yeast_extract
ing3_Agar["Agar"]
CultureMech_005291 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_KH2PO4["KH2PO4"]
CultureMech_005291 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_005291 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005291 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_NaCl["NaCl"]
CultureMech_005291 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
CultureMech_005291 --> ing8_MnSO4_x_5_H2O
CHEBI_131524["CHEBI:131524"]
ing8_MnSO4_x_5_H2O -.-> CHEBI_131524
ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_005291 --> ing9_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005291 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_Na3_citrate["Na3-citrate"]
CultureMech_005291 --> ing11_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing11_Na3_citrate -.-> CHEBI_53258
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Peptone | 10 G_PER_L | ||
| 3 | Yeast extract | 15 G_PER_L | ||
| 4 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 |
MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
|
1 G_PER_L |
CHEBI:131524
manganese(II) sulfate pentahydrate
|
|
| 10 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
20 G_PER_L |
CHEBI:53258
sodium citrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_452_GLUCOSE_medium_NAKAYAMA (CultureMech:005291)
- glucose_medium_nakayama (CultureMech:001562)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 452 |
| 2026-02-03T11:19:14.205254 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 452 |
| 2026-03-13T06:19:31.547313Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005291 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:47.442137+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GLUCOSE medium (NAKAYAMA)" to "glucose_medium_nakayama" for consistent matching |
| 2026-06-05T07:40:23.389506+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.913719+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:49.471928+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_452_GLUCOSE_medium_NAKAYAMA, glucose_medium_nakayama |
| 2026-08-06T03:04:49.471970+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 213f1b7538e2ec4094c6775a41882f369ec2478538a35d37579eefbd6a93c33e |