Overview
- External ontology
- mediadive.medium:452 (source link unavailable) — GLUCOSE MEDIUM (NAKAYAMA)
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium452.pdf
Composition
flowchart LR
CultureMech_001562["glucose_medium_nakayama"]
ing0_Glucose["Glucose"]
CultureMech_001562 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Peptone["Peptone"]
CultureMech_001562 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_001562 --> ing2_Yeast_extract
ing3_Agar["Agar"]
CultureMech_001562 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Distilled_water["Distilled water"]
CultureMech_001562 --> ing4_Distilled_water
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Peptone | 10 G_PER_L | ||
| 3 | Yeast extract | 15 G_PER_L | ||
| 4 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
|
| 5 | Distilled water | 0.98 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
Preparation
- Autoclave all solutions separately, final pH is 6.8.
Solutions
Solution A
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | 5 G_PER_L | |
| K2HPO4 | 5 G_PER_L | |
| Distilled water | 0.1 L |
Solution B
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7 H2O | 30 G_PER_L | |
| NaCl | 1 G_PER_L | |
| MnSO4 x 5 H2O | 1 G_PER_L | |
| CuSO4 x 5 H2O | 0.1 G_PER_L | |
| Distilled water | 0.1 L |
Solution C
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeSO4 x 7 H2O | 1 G_PER_L | |
| Na3-citrate | 20 G_PER_L | |
| Distilled water | 0.1 L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.163126Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 452 |
| 2026-03-13T06:19:05.199579Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001562 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.303137+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GLUCOSE MEDIUM (NAKAYAMA)" to "glucose_medium_nakayama" for consistent matching |
| 2026-06-05T07:41:06.440867+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.119875+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T11:35:03.558874+00:00 | codex | RESTORED_REVIEWED_MEDIUM_STOCK_BOUNDARIES | Exact source graph, complete ingredient inventory, amounts, and original PDF recipe chain checked. Numeric concentrations and chemical mappings preserved; stock doses and water are explicit batch amounts. No final-medium stock dilution inferred. Parent/variant relationships remain separately unreviewed. |