Overview
- External ontology
- komodo.medium:473 (source link unavailable) — MEDIUM WITH EDTA AS CARBON SOURCE
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_473_modified_for_dsm_6780', 'source': 'KOMODO', 'source_id': 'komodo.medium:473_6780', 'original_category': 'bacterial'}
- pH range
- {'min': 7.5, 'max': 7.6}
- Notes
- Source: KOMODO ModelSEED | ID: 473 | DSMZ Medium: 473 (mediadive.medium:473) | Aerobic: No
Composition
flowchart LR
CultureMech_005588["medium_with_edta_as_carbon_source"]
ing0_K2HPO4["K2HPO4"]
CultureMech_005588 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_005588 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_005588 --> ing2_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing2_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005588 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_005588 --> ing4_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing4_Ferric_ammonium_citrate -.-> CHEBI_31604
ing5_Na2_EDTA["Na2-EDTA"]
CultureMech_005588 --> ing5_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing5_Na2_EDTA -.-> CHEBI_64734
ing6_Agar["Agar"]
CultureMech_005588 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Biotin["Biotin"]
CultureMech_005588 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Folic_acid["Folic acid"]
CultureMech_005588 --> ing8_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing8_Folic_acid -.-> CHEBI_27470
ing9_Thiamine_HCl["Thiamine HCl"]
CultureMech_005588 --> ing9_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing9_Thiamine_HCl -.-> CHEBI_49105
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_005588 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005588 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_005588 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005588 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005588 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005588 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005588 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_p_Aminobenzoate["p-Aminobenzoate"]
CultureMech_005588 --> ing17_p_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing17_p_Aminobenzoate -.-> CHEBI_17836
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_005588 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_005588 --> ing19_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing19_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing20_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_005588 --> ing20_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_pantothenate -.-> CHEBI_31345
ing21_Pyridoxamine["Pyridoxamine"]
CultureMech_005588 --> ing21_Pyridoxamine
CHEBI_16410["CHEBI:16410"]
ing21_Pyridoxamine -.-> CHEBI_16410
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_005588 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.272 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.02 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.49 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Ferric ammonium citrate | 0.08 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 6 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.2 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.802 G_PER_L |
CHEBI:15956
biotin
|
[Merged 2 duplicates: 0.8, 0.002] |
| 9 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.8 G_PER_L |
CHEBI:27470
folic acid
|
|
| 10 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.8 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
p-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.01 G_PER_L |
CHEBI:17836
4-aminobenzoate
|
|
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.02 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 20 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 21 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 |
Pyridoxamine
C8H12N2O2 168.196 g/mol
|
0.05 G_PER_L |
CHEBI:16410
pyridoxamine
|
|
| 23 | Vitamin B12 | 0.02 G_PER_L |
CHEBI:176843
vitamin B12
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_473_MEDIUM_WITH_EDTA_AS_CARBON_SOURCE (CultureMech:005588)
- medium_473_modified_for_dsm_6780 (CultureMech:005587)
- medium_with_edta_as_carbon_source (CultureMech:001602)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 473 |
| 2026-02-03T11:19:07.426525 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 473 |
| 2026-03-13T03:09:11.970625Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:34.334029Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005588 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:50.554948+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM WITH EDTA AS CARBON SOURCE" to "medium_with_edta_as_carbon_source" for consistent matching |
| 2026-05-20T06:00:52.184699+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.5-7.6' -> {'min': 7.5, 'max': 7.6} |
| 2026-06-05T07:40:23.905775+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.091893+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:53.112777+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_473_MEDIUM_WITH_EDTA_AS_CARBON_SOURCE, medium_473_modified_for_dsm_6780, medium_with_edta_as_carbon_source |
| 2026-08-06T03:04:53.112830+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 5622c375c20628fffa2d5353bc3162cb921cd17c25c19ce72fd4621c94a37d89 |