medium_with_edta_as_carbon_source

CultureMech:001602 bacterial DEFINED SOLID_AGAR
Original name: MEDIUM WITH EDTA AS CARBON SOURCE

Overview

External ontology
mediadive.medium:473 (source link unavailable) — MEDIUM WITH EDTA AS CARBON SOURCE
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 7.6}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium473.pdf

Composition

flowchart LR
    CultureMech_001602["medium_with_edta_as_carbon_source"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001602 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001602 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_001602 --> ing2_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing2_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001602 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_001602 --> ing4_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing4_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_001602 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_Agar["Agar"]
    CultureMech_001602 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Biotin["Biotin"]
    CultureMech_001602 --> ing7_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing7_Biotin -.-> CHEBI_15956
    ing8_Folic_acid["Folic acid"]
    CultureMech_001602 --> ing8_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing8_Folic_acid -.-> CHEBI_27470
    ing9_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001602 --> ing9_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing9_Thiamine_HCl -.-> CHEBI_49105
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001602 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001602 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_001602 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001602 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001602 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001602 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001602 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_p_Aminobenzoate["p-Aminobenzoate"]
    CultureMech_001602 --> ing17_p_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing17_p_Aminobenzoate -.-> CHEBI_17836
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001602 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001602 --> ing19_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing19_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001602 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_Pyridoxamine["Pyridoxamine"]
    CultureMech_001602 --> ing21_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing21_Pyridoxamine -.-> CHEBI_16410
    ing22_Vitamin_B12["Vitamin B12"]
    CultureMech_001602 --> ing22_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing22_Vitamin_B12 -.-> CHEBI_176843

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
1.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.272 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.02 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.49 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Ferric ammonium citrate 0.08 G_PER_L CHEBI:31604
ferric ammonium citrate
6 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
7 Agar 15 G_PER_L CHEBI:2509
agar
8 Biotin
C10H16N2O3S 244.316 g/mol
0.802 G_PER_L CHEBI:15956
biotin
 [Merged 2 duplicates: 0.8, 0.002]
9 Folic acid
C19H19N7O6 441.404 g/mol
0.8 G_PER_L CHEBI:27470
folic acid
10 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.8 G_PER_L CHEBI:49105
thiamine hydrochloride
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 p-Aminobenzoate
C7H6NO2 136.13 g/mol
0.01 G_PER_L CHEBI:17836
4-aminobenzoate
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
20 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
21 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
22 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.05 G_PER_L CHEBI:16410
pyridoxamine
23 Vitamin B12 0.02 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Autoclave phosphate buffer separately. pH should be 7.5 - 7.6. Add 5.0 ml sterile stock solution (see medium 462).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.284294Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 473
2026-03-13T03:09:40.436062Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:05.564743Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001602
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.697280+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM WITH EDTA AS CARBON SOURCE" to "medium_with_edta_as_carbon_source" for consistent matching
2026-05-20T06:01:54.800153+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.5-7.6' -> {'min': 7.5, 'max': 7.6}
2026-06-05T07:41:30.332707+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.529043+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.