Overview
- External ontology
- komodo.medium:474 (source link unavailable) — MEDIUM WITH POLYHYDROXYBUTYRIC ACID AS CARBON SOURCE
- Applications
-
- Microbial cultivation
- Notes
- Source: KOMODO ModelSEED | ID: 474 | DSMZ Medium: 474 (mediadive.medium:474) | Aerobic: No Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_005589["medium_with_polyhydroxybutyric_acid_as_carbon_source"]
ing0_Agar["Agar"]
CultureMech_005589 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1_poly___hydroxybutyric_acid["poly-ß-hydroxybutyric acid"]
CultureMech_005589 --> ing1_poly___hydroxybutyric_acid
ing2_Na2HPO4["Na2HPO4"]
CultureMech_005589 --> ing2_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4 -.-> CHEBI_34683
ing3_KH2PO4["KH2PO4"]
CultureMech_005589 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_005589 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005589 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005589 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_EDTA["EDTA"]
CultureMech_005589 --> ing7_EDTA
CHEBI_4735["CHEBI:4735"]
ing7_EDTA -.-> CHEBI_4735
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005589 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_005589 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005589 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_005589 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005589 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005589 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005589 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005589 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_Peptone["Peptone"]
CultureMech_005589 --> ing16_Peptone
ing17_Meat_extract["Meat extract"]
CultureMech_005589 --> ing17_Meat_extract
ing18_Casein_peptone["Casein peptone"]
CultureMech_005589 --> ing18_Casein_peptone
ing19_Soy_peptone["Soy peptone"]
CultureMech_005589 --> ing19_Soy_peptone
ing20_NaCl["NaCl"]
CultureMech_005589 --> ing20_NaCl
CHEBI_26710["CHEBI:26710"]
ing20_NaCl -.-> CHEBI_26710
ing21_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_005589 --> ing21_Pancreatic_digest_of_casein
ing22_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_005589 --> ing22_Peptic_digest_of_soybean_meal
ing23_Glucose["Glucose"]
CultureMech_005589 --> ing23_Glucose
CHEBI_17234["CHEBI:17234"]
ing23_Glucose -.-> CHEBI_17234
ing24_Sodium_chloride["Sodium chloride"]
CultureMech_005589 --> ing24_Sodium_chloride
ing24_Sodium_chloride -.-> CHEBI_26710
ing25_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_005589 --> ing25_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing25_Dipotassium_phosphate -.-> CHEBI_131527
ing26_Agar["Agar"]
CultureMech_005589 --> ing26_Agar
ing26_Agar -.-> CHEBI_2509
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 66.0 G_PER_L |
CHEBI:2509
agar
|
[Merged 4 duplicates: 16.0, 15.0, 15.0, 20.0] |
| 2 | poly-ß-hydroxybutyric acid | 30 G_PER_L | ||
| 3 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
2.44 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.52 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 | Peptone | 5 G_PER_L | ||
| 18 | Meat extract | 3 G_PER_L | ||
| 19 | Casein peptone | 15 G_PER_L | ||
| 20 | Soy peptone | 5 G_PER_L | ||
| 21 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 22 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 23 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 24 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 25 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 26 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 27 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_474_MEDIUM_WITH_POLYHYDROXYBUTYRIC_ACID_AS_CARBON_SOURCE (CultureMech:005589)
- medium_with_polyhydroxybutyric_acid_as_carbon_source (CultureMech:001603)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 474 |
| 2026-02-03T11:19:19.577492 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 474 |
| 2026-03-13T03:09:43.957083Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:34.344044Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005589 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:50.566249+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM WITH POLYHYDROXYBUTYRIC ACID AS CARBON SOURCE" to "medium_with_polyhydroxybutyric_acid_as_carbon_source" for consistent matching |
| 2026-06-05T07:40:23.922400+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.097614+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:53.144438+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_474_MEDIUM_WITH_POLYHYDROXYBUTYRIC_ACID_AS_CARBON_SOURCE, medium_with_polyhydroxybutyric_acid_as_carbon_source |
| 2026-08-06T03:04:53.144499+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 0c782bce7d5ed6e5c34340e19bfdbe758fa87c2fcff313515c7752ea88487404 |