medium_with_polyhydroxybutyric_acid_as_carbon_source

Overview

External ontology

mediadive.medium:474 (source link unavailable) — MEDIUM WITH POLYHYDROXYBUTYRIC ACID AS CARBON SOURCE

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium474.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001603["medium_with_polyhydroxybutyric_acid_as_carbon_source"]
    ing0_Agar["Agar"]
    CultureMech_001603 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_poly___hydroxybutyric_acid["poly-ß-hydroxybutyric acid"]
    CultureMech_001603 --> ing1_poly___hydroxybutyric_acid
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_001603 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001603 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001603 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001603 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001603 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_EDTA["EDTA"]
    CultureMech_001603 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001603 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001603 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001603 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_001603 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001603 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001603 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001603 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001603 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_Peptone["Peptone"]
    CultureMech_001603 --> ing16_Peptone
    ing17_Meat_extract["Meat extract"]
    CultureMech_001603 --> ing17_Meat_extract
    ing18_Casein_peptone["Casein peptone"]
    CultureMech_001603 --> ing18_Casein_peptone
    ing19_Soy_peptone["Soy peptone"]
    CultureMech_001603 --> ing19_Soy_peptone
    ing20_NaCl["NaCl"]
    CultureMech_001603 --> ing20_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing20_NaCl -.-> CHEBI_26710
    ing21_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001603 --> ing21_Pancreatic_digest_of_casein
    ing22_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001603 --> ing22_Peptic_digest_of_soybean_meal
    ing23_Glucose["Glucose"]
    CultureMech_001603 --> ing23_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing23_Glucose -.-> CHEBI_17234
    ing24_Sodium_chloride["Sodium chloride"]
    CultureMech_001603 --> ing24_Sodium_chloride
    ing24_Sodium_chloride -.-> CHEBI_26710
    ing25_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001603 --> ing25_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing25_Dipotassium_phosphate -.-> CHEBI_131527
    ing26_Agar["Agar"]
    CultureMech_001603 --> ing26_Agar
    ing26_Agar -.-> CHEBI_2509

Ingredients (27)

Preparation

1. Prepare a suspension of 3.0 g poly-ß-hydroxybutyric acid (PHB) in 100 ml distilled water, stir over night and sonicate until a white homogenous suspension is obtained. Autoclave for 5 minutes at 121°C. Prepare double layer agar plates. First layer: medium 457 with 16.0 g agar per l.
2. Second layer: medium 457 with agar, supplemented with 6.6 ml sterile PHB-suspension per 100 ml.
3. Adjust pH to 6.9.
4. Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling.
5. Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source.
6. Adjust pH to 7.0.
7. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
8. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
9. pH 7.3
10. Autoclave at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance