Overview
- External ontology
- komodo.medium:546 (source link unavailable) — LC BROTH medium
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: KOMODO ModelSEED | ID: 546 | DSMZ Medium: 546 (mediadive.medium:546) | Aerobic: No
Composition
flowchart LR
CultureMech_005987["lc_broth_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_005987 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_005987 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_005987 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Glucose["Glucose"]
CultureMech_005987 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005987 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005987 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.735075 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.23238 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_546_LC_BROTH_medium (CultureMech:005987)
- lc_broth_medium (CultureMech:001680)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 546 |
| 2026-02-03T11:19:15.640475 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 546 |
| 2026-03-13T06:19:37.474278Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005987 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:54.251561+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LC BROTH medium" to "lc_broth_medium" for consistent matching |
| 2026-06-05T07:40:24.547153+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:55.407150+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_546_LC_BROTH_medium, lc_broth_medium |
| 2026-08-06T03:04:55.407193+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 75892697759ab2ef7c0a8e47a5173d1d5d6a68c30f5d72034a40434b378ef30e |