Overview
- External ontology
- komodo.medium:546 (source link unavailable) — LC BROTH medium
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: KOMODO ModelSEED | ID: 546 | DSMZ Medium: 546 (mediadive.medium:546) | Aerobic: No Stock follow-up: quantities distinguish base-water inputs, stock strengths and batch doses; no measured final volume or new growth claim is inferred. Existing chemical mappings and variant links are retained, not independently validated.
Composition
flowchart LR
CultureMech_005987["lc_broth_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_005987 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_005987 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_005987 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Glucose["Glucose"]
CultureMech_005987 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Distilled_water["Distilled water"]
CultureMech_005987 --> ing4_Distilled_water
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L | Source batch input: 10 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. | |
| 2 | Yeast extract | 5 G_PER_L | Source batch input: 5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Source batch input: 10 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Source batch input: 1 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 5 | Distilled water | 0.99 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
Distilled-water input to the base per recipe batch, not final medium volume or stock carrier volume. |
Preparation
- Adjust pH to 7.4.
- Sterilize at 121°C for 15 min. Cool to 50°C and add 5 ml 1 M CaCl2 x 2 H2O and 5 ml 1 M MgSO4 x 7 H2O as sterile solutions.
Solutions
CaCl2 x 2 H2O stock
Dose: 0.005 L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2 H2O | 1 MOLAR | Source-explicit stock strength, not a final-medium concentration. The former flattened value is replaced at the stock stage; no molecular-weight or density conversion is inferred. |
5 ml stock addition per recipe batch, not per liter of an inferred final medium. Carrier amount and final-medium concentration are not inferred.
MgSO4 x 7 H2O stock
Dose: 0.005 L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7 H2O | 1 MOLAR | Source-explicit stock strength, not a final-medium concentration. The former flattened value is replaced at the stock stage; no molecular-weight or density conversion is inferred. |
5 ml stock addition per recipe batch, not per liter of an inferred final medium. Carrier amount and final-medium concentration are not inferred.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 546 |
| 2026-02-03T11:19:15.640475 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 546 |
| 2026-03-13T06:19:37.474278Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005987 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:54.251561+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LC BROTH medium" to "lc_broth_medium" for consistent matching |
| 2026-06-05T07:40:24.547153+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-08T04:59:45.451604+00:00 | codex | CORRECTED_DSMZ546_659_670_STOCK_STAGES | Restored source-explicit stock strengths/doses and base-water inputs; prior excerpts, history and mappings preserved. No strain-variant or growth curation is claimed. |