m13_verrucomicrobium_medium

CultureMech:006096 bacterial COMPLEX LIQUID
Original name: M13 VERRUCOMICROBIUM medium

Overview

External ontology
komodo.medium:607 (source link unavailable) — M13 VERRUCOMICROBIUM medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'filobacillus_milosensis_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:607a', 'original_category': 'bacterial'}
  • {'name': 'filobacillus_milosensis_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:607a', 'original_category': 'bacterial'}
pH
6.8
Notes
Source: KOMODO ModelSEED | ID: 607 | DSMZ Medium: 607 (mediadive.medium:607) | Aerobic: No

Composition

flowchart LR
    CultureMech_006096["m13_verrucomicrobium_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_006096 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_006096 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_006096 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_006096 --> ing3_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing3_Tris_HCl_buffer -.-> CHEBI_9754
    ing4_NaCl["NaCl"]
    CultureMech_006096 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_006096 --> ing5_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006096 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006096 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_006096 --> ing8__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing8__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_006096 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_006096 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_Biotin["Biotin"]
    CultureMech_006096 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_006096 --> ing12_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing12_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing13_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_006096 --> ing13_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing13_Calcium_pantothenate -.-> CHEBI_31345
    ing14_Folic_acid["Folic acid"]
    CultureMech_006096 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Riboflavin["Riboflavin"]
    CultureMech_006096 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinamide["Nicotinamide"]
    CultureMech_006096 --> ing16_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing16_Nicotinamide -.-> CHEBI_17154
    ing17_Na2SO4["Na2SO4"]
    CultureMech_006096 --> ing17_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing17_Na2SO4 -.-> CHEBI_32149
    ing18_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006096 --> ing18_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing18_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing19_CaCl2["CaCl2"]
    CultureMech_006096 --> ing19_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing19_CaCl2 -.-> CHEBI_3312
    ing20_NaHCO3["NaHCO3"]
    CultureMech_006096 --> ing20_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing20_NaHCO3 -.-> CHEBI_32139
    ing21_KCl["KCl"]
    CultureMech_006096 --> ing21_KCl
    CHEBI_32588["CHEBI:32588"]
    ing21_KCl -.-> CHEBI_32588
    ing22_KBr["KBr"]
    CultureMech_006096 --> ing22_KBr
    CHEBI_32030["CHEBI:32030"]
    ing22_KBr -.-> CHEBI_32030
    ing23_H3BO3["H3BO3"]
    CultureMech_006096 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_SrCl2["SrCl2"]
    CultureMech_006096 --> ing24_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing24_SrCl2 -.-> CHEBI_36383
    ing25_NaF["NaF"]
    CultureMech_006096 --> ing25_NaF
    CHEBI_28741["CHEBI:28741"]
    ing25_NaF -.-> CHEBI_28741
    ing26_Na_EDTA["Na-EDTA"]
    CultureMech_006096 --> ing26_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing26_Na_EDTA -.-> CHEBI_64734
    ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_006096 --> ing27_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing28_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_006096 --> ing28_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing28_MnSO4_x_H2O -.-> CHEBI_86364
    ing29_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_006096 --> ing29_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing29_CuSO4_x_5_H2O -.-> CHEBI_31440
    CultureMech_006096 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 Peptone 0.75 G_PER_L
2 Yeast extract 0.75 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
0.75 G_PER_L CHEBI:17234
glucose
4 Tris-HCl buffer
C4H11NO3 121.136 g/mol
50 G_PER_L CHEBI:9754
tris
5 NaCl
Cl.Na 58.443 g/mol
123.477 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 100.0, 23.477]
6 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
11 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
12 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
13 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
14 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
15 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
17 Nicotinamide
C6H6N2O 122.127 g/mol
0.005 G_PER_L CHEBI:17154
nicotinamide
18 Na2SO4
2Na.O4S 142.043 g/mol
3.917 G_PER_L CHEBI:32149
sodium sulfate
19 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.981 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
20 CaCl2
Ca.2Cl 110.984 g/mol
1.102 G_PER_L CHEBI:3312
calcium dichloride
21 NaHCO3
CHO3.Na 84.006 g/mol
0.192 G_PER_L CHEBI:32139
sodium hydrogencarbonate
22 KCl
Cl.K 74.551 g/mol
0.664 G_PER_L CHEBI:32588
potassium chloride
23 KBr
Br.K 119.002 g/mol
0.006 G_PER_L CHEBI:32030
potassium bromide
24 H3BO3
H3BO3 61.833 g/mol
0.026 G_PER_L CHEBI:33118
boric acid
25 SrCl2
2Cl.Sr 158.526 g/mol
0.024 G_PER_L CHEBI:36383
strontium dichloride
26 NaF
F.Na 41.988 g/mol
0.003 G_PER_L CHEBI:28741
sodium fluoride
27 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
28 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
29 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
30 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
31 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
32 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 607
2026-02-03T11:18:59.105458 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 607
2026-03-13T03:08:49.660660Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:38.234304Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006096
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:55.126047+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M13 VERRUCOMICROBIUM medium" to "m13_verrucomicrobium_medium" for consistent matching
2026-06-05T07:40:24.909791+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:45.377353+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:56.125611+00:00 recipe-merger Merged 4 duplicate recipes into canonical record Sources: KOMODO_607_M13_VERRUCOMICROBIUM_medium, KOMODO_607a_FILOBACILLUS_MILOSENSIS_medium, filobacillus_milosensis_medium, m13_verrucomicrobium_medium
2026-08-06T03:04:56.125687+00:00 merge_recipes.py MERGED_RECIPES merged 4 source recipe(s) on fingerprint de47b12db78e29adaff1e02126d6cfcfcda16e456eebb112e3f5937978d42c86