Overview
- External ontology
- komodo.medium:629 (source link unavailable) — STALEY'S MAINTENANCE medium
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: KOMODO ModelSEED | ID: 629 | DSMZ Medium: 629 (mediadive.medium:629) | Aerobic: No
Composition
flowchart LR
CultureMech_006135["staleys_maintenance_medium"]
ing0_Peptone["Peptone"]
CultureMech_006135 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_006135 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_006135 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_006135 --> ing3_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing3_Nitrilotriacetic_acid -.-> CHEBI_44557
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006135 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006135 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_006135 --> ing6__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing6__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006135 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_Vitamin_B12["Vitamin B12"]
CultureMech_006135 --> ing8_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing8_Vitamin_B12 -.-> CHEBI_176843
ing9_Biotin["Biotin"]
CultureMech_006135 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_006135 --> ing10_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing10_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing11_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006135 --> ing11_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing11_Calcium_pantothenate -.-> CHEBI_31345
ing12_Folic_acid["Folic acid"]
CultureMech_006135 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Riboflavin["Riboflavin"]
CultureMech_006135 --> ing13_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing13_Riboflavin -.-> CHEBI_17015
ing14_Nicotinamide["Nicotinamide"]
CultureMech_006135 --> ing14_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing14_Nicotinamide -.-> CHEBI_17154
ing15_Na_EDTA["Na-EDTA"]
CultureMech_006135 --> ing15_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing15_Na_EDTA -.-> CHEBI_64734
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006135 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_006135 --> ing17_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing17_MnSO4_x_H2O -.-> CHEBI_86364
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006135 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_006135 --> ing19_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing19_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing20_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_006135 --> ing20_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing20_Na2B4O7_x_10_H2O -.-> CHEBI_131366
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 4 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.335 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.00925 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.599 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.099, 0.5] |
| 9 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 12 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 15 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.009 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 16 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.154 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0392 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.0248 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 21 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_629_STALEY_S_MAINTENANCE_medium (CultureMech:006135)
- staleys_maintenance_medium (CultureMech:001760)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 629 |
| 2026-02-03T11:18:56.992542 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 629 |
| 2026-03-13T03:08:44.214868Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:38.538856Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006135 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:55.476768+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "STALEY'S MAINTENANCE medium" to "staleys_maintenance_medium" for consistent matching |
| 2026-06-05T07:40:25.063680+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.421320+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:56.404328+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_629_STALEY_S_MAINTENANCE_medium, staleys_maintenance_medium |
| 2026-08-06T03:04:56.404362+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint f030b272dba8d1dcb0d35ff0073aa4c9e7a629e65cc3e39ba0577e3415809305 |