staleys_maintenance_medium

CultureMech:001760 bacterial COMPLEX SOLID_AGAR
Original name: STALEY'S MAINTENANCE MEDIUM

Overview

External ontology
mediadive.medium:629 (source link unavailable) — STALEY'S MAINTENANCE MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium629.pdf

Composition

flowchart LR
    CultureMech_001760["staleys_maintenance_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_001760 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001760 --> ing1_Yeast_extract
    ing2_Agar["Agar"]
    CultureMech_001760 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001760 --> ing3_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing3_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001760 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001760 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001760 --> ing6__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing6__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001760 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_Vitamin_B12["Vitamin B12"]
    CultureMech_001760 --> ing8_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing8_Vitamin_B12 -.-> CHEBI_176843
    ing9_Biotin["Biotin"]
    CultureMech_001760 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001760 --> ing10_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing10_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing11_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001760 --> ing11_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing11_Calcium_pantothenate -.-> CHEBI_31345
    ing12_Folic_acid["Folic acid"]
    CultureMech_001760 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Riboflavin["Riboflavin"]
    CultureMech_001760 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Nicotinamide["Nicotinamide"]
    CultureMech_001760 --> ing14_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing14_Nicotinamide -.-> CHEBI_17154
    ing15_Na_EDTA["Na-EDTA"]
    CultureMech_001760 --> ing15_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing15_Na_EDTA -.-> CHEBI_64734
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001760 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001760 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001760 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001760 --> ing19_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing19_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing20_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001760 --> ing20_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing20_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L
2 Yeast extract 0.5 G_PER_L
3 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
4 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
9 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
10 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
11 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
12 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
13 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
14 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
15 Nicotinamide
C6H6N2O 122.127 g/mol
0.009 G_PER_L CHEBI:17154
nicotinamide
16 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
21 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. Dissolve peptone and yeast extract in the distilled water, adjust the pH to 7.2 and sterilize. After the medium has cooled add the Hutner's salts and Vitamin solution.
  2. For solid medium use 15.0 g agar.
  3. Dissolve the nitrilotriacetic acid.
  4. Adjust pH to 7.0 with KOH (~7.3 g).
  5. Dissolve other salts separately and combine.
  6. Adjust pH to 6.8 with NaOH or H2SO4.
  7. Filter-sterilized.
  8. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.776443Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 629
2026-03-13T03:08:44.502363Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:07.018068Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001760
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.270422+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "STALEY'S MAINTENANCE MEDIUM" to "staleys_maintenance_medium" for consistent matching
2026-06-05T07:41:46.918947+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.807986+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.