mineral_medium_for_chemolithotrophic_growth_h_3

CultureMech:006545 bacterial DEFINED SOLID_AGAR
Original name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Overview

External ontology
komodo.medium:81 (source link unavailable) — MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'for_heterotrophic_growth', 'source': 'KOMODO', 'source_id': 'komodo.medium:81.3', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_21436', 'source': 'KOMODO', 'source_id': 'komodo.medium:81.4', 'original_category': 'bacterial'}
  • {'name': 'for_growth_on_nitrogen_free_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:81.2', 'original_category': 'bacterial'}
  • {'name': 'for_heterotrophic_growth', 'source': 'KOMODO', 'source_id': 'komodo.medium:81.1', 'original_category': 'bacterial'}
  • {'name': 'medium_81_modified_for_dsm_21442', 'source': 'KOMODO', 'source_id': 'komodo.medium:81_21442', 'original_category': 'bacterial'}
pH
6.8
Notes
Source: KOMODO ModelSEED | ID: 81 | DSMZ Medium: 81 (mediadive.medium:81) | Aerobic: No

Composition

flowchart LR
    CultureMech_006545["mineral_medium_for_chemolithotrophic_growth_h_3"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_006545 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_006545 --> ing1_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing2_NH4Cl["NH4Cl"]
    CultureMech_006545 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006545 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006545 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006545 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_NaVO3_x_H2O["NaVO3 x H2O"]
    CultureMech_006545 --> ing6_NaVO3_x_H2O
    CHEBI_132095["CHEBI:132095"]
    ing6_NaVO3_x_H2O -.-> CHEBI_132095
    ing7_Agar["Agar"]
    CultureMech_006545 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_006545 --> ing8_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing8_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing9_Riboflavin["Riboflavin"]
    CultureMech_006545 --> ing9_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing9_Riboflavin -.-> CHEBI_17015
    ing10_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_006545 --> ing10_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing10_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing11_Nicotinic_acid["Nicotinic acid"]
    CultureMech_006545 --> ing11_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing11_Nicotinic_acid -.-> CHEBI_15940
    ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_006545 --> ing12_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing13_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_006545 --> ing13_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing13_Calcium_pantothenate -.-> CHEBI_31345
    ing14_Biotin["Biotin"]
    CultureMech_006545 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_Folic_acid["Folic acid"]
    CultureMech_006545 --> ing15_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing15_Folic_acid -.-> CHEBI_27470
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_006545 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_006545 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_H3BO3["H3BO3"]
    CultureMech_006545 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006545 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006545 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006545 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006545 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
46 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
58 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
1.08696 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.543478 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0108696 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03543478 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Merged 2 duplicates: 0.00543478, 0.03]
7 NaVO3 x H2O
H2O.Na.O3V 139.946 g/mol
0.00543478 G_PER_L CHEBI:132095
sodium metavanadate hydrate
8 Agar 21.7391 G_PER_L CHEBI:2509
agar
 (if required)
9 Ferric ammonium citrate 2.5 G_PER_L CHEBI:31604
ferric ammonium citrate
10 Riboflavin
C17H20N4O6 376.369 g/mol
0.1 G_PER_L CHEBI:17015
riboflavin
11 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.5 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
12 Nicotinic acid
C6H5NO2 123.111 g/mol
0.5 G_PER_L CHEBI:15940
nicotinic acid
13 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.5 G_PER_L CHEBI:30961
pyridoxine hydrochloride
14 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.5 G_PER_L CHEBI:31345
Calcium pantothenate
15 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
16 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
17 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Target organisms

No target organisms are recorded.

Provenance

Merged from 7 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 81
2026-02-03T11:19:08.698357 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 81
2026-03-13T03:09:14.921535Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:41.745746Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006545
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:59.250881+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)" to "mineral_medium_for_chemolithotrophic_growth_h_3" for consistent matching
2026-06-05T07:40:26.728774+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:45.988656+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:59.701021+00:00 recipe-merger Merged 7 duplicate recipes into canonical record Sources: KOMODO_81-3_For_heterotrophic_growth, KOMODO_81_MINERAL_MEDIUM_FOR_CHEMOLITHOTROPHIC_GROWTH_H-3, for_dsm_21436, for_growth_on_nitrogen_free_medium, for_heterotrophic_growth, medium_81_modified_for_dsm_21442, mineral_medium_for_chemolithotrophic_growth_h_3
2026-08-06T03:04:59.701118+00:00 merge_recipes.py MERGED_RECIPES merged 7 source recipe(s) on fingerprint 1d329f4b25be39579d1a78f2195b99f408d0a0ea2ea597c278a1aa372b02a091