Overview
- External ontology
- komodo.medium:81.1 (source link unavailable) — For heterotrophic growth
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: KOMODO ModelSEED | ID: 81.1 | DSMZ Medium: 81 (mediadive.medium:81) | Aerobic: No
Composition
flowchart LR
CultureMech_006528["for_heterotrophic_growth"]
ing0_KH2PO4["KH2PO4"]
CultureMech_006528 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_006528 --> ing1_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing2_NH4Cl["NH4Cl"]
CultureMech_006528 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006528 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006528 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006528 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_NaVO3_x_H2O["NaVO3 x H2O"]
CultureMech_006528 --> ing6_NaVO3_x_H2O
CHEBI_132095["CHEBI:132095"]
ing6_NaVO3_x_H2O -.-> CHEBI_132095
ing7_Agar["Agar"]
CultureMech_006528 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_006528 --> ing8_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing8_Ferric_ammonium_citrate -.-> CHEBI_31604
ing9_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006528 --> ing9_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing9_Calcium_pantothenate -.-> CHEBI_31345
ing10_Biotin["Biotin"]
CultureMech_006528 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_006528 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_006528 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006528 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_H3BO3["H3BO3"]
CultureMech_006528 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006528 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006528 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006528 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006528 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
46 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
58 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.08696 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.543478 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0108696 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03543478 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
[Merged 2 duplicates: 0.00543478, 0.03] |
| 7 |
NaVO3 x H2O
H2O.Na.O3V 139.946 g/mol
|
0.00543478 G_PER_L |
CHEBI:132095
sodium metavanadate hydrate
|
|
| 8 | Agar | 21.7391 G_PER_L |
CHEBI:2509
agar
|
(if required) |
| 9 | Ferric ammonium citrate | 2.5 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 10 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Solutions
Standard vitamin solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Riboflavin | 0.1 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.5 G_PER_L | |
| Nicotinic acid | 0.5 G_PER_L | |
| Pyridoxine hydrochloride | 0.5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 81.1 |
| 2026-02-03T11:19:18.727925 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 81 |
| 2026-03-13T03:09:41.672420Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:41.565614Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006528 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:59.043719+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For heterotrophic growth" to "for_heterotrophic_growth" for consistent matching |
| 2026-06-05T07:41:05.110433+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.676291+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:30.918689+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Standard vitamin solution' @ 5 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |