medium_r2a_for_denitrifying_bacteria

CultureMech:006621 bacterial COMPLEX LIQUID
Original name: medium R2A FOR DENITRIFYING BACTERIA

Overview

External ontology
komodo.medium:830b (source link unavailable) — medium R2A FOR DENITRIFYING BACTERIA
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'r2a_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:830', 'original_category': 'bacterial'}
  • {'name': 'medium_r2a_modified', 'source': 'KOMODO', 'source_id': 'komodo.medium:830a', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_22224', 'source': 'KOMODO', 'source_id': 'komodo.medium:830.1', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_13665', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_13665', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_14773', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_14773', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_14805', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_14805', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_15671', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_15671', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_16968', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_16968', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_16969', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_16969', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_17871', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_17871', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18042', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18042', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18043', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18043', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18045', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18045', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18135', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18135', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18203', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18203', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18221', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18221', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18603', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18603', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18704', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18704', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_18758', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_18758', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_19096', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_19096', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_19249', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_19249', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_19250', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_19250', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_19309', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_19309', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_19717', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_19717', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_19724', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_19724', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21036', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21036', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21240', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21240', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21316', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21316', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21346', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21346', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21362', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21362', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21490', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21490', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21568', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21568', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21789', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21789', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21942', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21942', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_21994', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_21994', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_22342', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_22342', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_22349', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_22349', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_22405', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_22405', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_22464', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_22464', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_22489', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_22489', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_22489_replace_glucose_with_fructose', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_22489_replace_Glucose_with_fructose', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_22869', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_22869', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_23119', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_23119', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_23228', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_23228', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_23491', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_23491', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_23886', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_23886', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_23906', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_23906', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_24448', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_24448', 'original_category': 'bacterial'}
  • {'name': 'medium_830_modified_for_dsm_25262', 'source': 'KOMODO', 'source_id': 'komodo.medium:830_25262', 'original_category': 'bacterial'}
  • {'name': 'medium_830a_modified_for_dsm_15637', 'source': 'KOMODO', 'source_id': 'komodo.medium:830a_15637', 'original_category': 'bacterial'}
  • {'name': 'medium_830c_modified_for_dsm_13663', 'source': 'KOMODO', 'source_id': 'komodo.medium:830c_13663', 'original_category': 'bacterial'}
  • {'name': 'medium_830c_modified_for_dsm_25221', 'source': 'KOMODO', 'source_id': 'komodo.medium:830c_25221', 'original_category': 'bacterial'}
Notes
Source: KOMODO ModelSEED | ID: 830b | DSMZ Medium: 830b (mediadive.medium:830b) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_006621["medium_r2a_for_denitrifying_bacteria"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_006621 --> ing0_Yeast_extract
    ing1_Proteose_peptone["Proteose peptone"]
    CultureMech_006621 --> ing1_Proteose_peptone
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_006621 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_006621 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_006621 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_006621 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_006621 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006621 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_KNO3["KNO3"]
    CultureMech_006621 --> ing8_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing8_KNO3 -.-> CHEBI_63043
    ing9_HCl["HCl"]
    CultureMech_006621 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_006621 --> ing10_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing11_ZnCl2["ZnCl2"]
    CultureMech_006621 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006621 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_006621 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006621 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006621 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006621 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006621 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.5 G_PER_L
2 Proteose peptone 0.5 G_PER_L
3 Casamino acids 0.5 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
5 Starch 0.5 G_PER_L CHEBI:28017
starch
6 Na-pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 KNO3
K.NO3 101.102 g/mol
0.5 G_PER_L CHEBI:63043
potassium nitrate
10 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
11 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Target organisms

No target organisms are recorded.

Provenance

Merged from 54 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 830b
2026-02-03T11:19:12.843628 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 830
2026-03-13T06:19:42.428905Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006621
2026-03-17T06:30:35.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:00.050096+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "medium R2A FOR DENITRIFYING BACTERIA" to "medium_r2a_for_denitrifying_bacteria" for consistent matching
2026-06-05T07:40:26.906001+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:28.662009+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:37:34.352199+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:46.053533+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:00.480405+00:00 recipe-merger Merged 54 duplicate recipes into canonical record Sources: KOMODO_830_R2A_medium, KOMODO_830a_medium_R2A_MODIFIED, KOMODO_830b_medium_R2A_FOR_DENITRIFYING_BACTERIA, for_dsm_22224, medium_830_modified_for_dsm_13665, medium_830_modified_for_dsm_14773, medium_830_modified_for_dsm_14805, medium_830_modified_for_dsm_15671, medium_830_modified_for_dsm_16968, medium_830_modified_for_dsm_16969, medium_830_modified_for_dsm_17871, medium_830_modified_for_dsm_18042, medium_830_modified_for_dsm_18043, medium_830_modified_for_dsm_18045, medium_830_modified_for_dsm_18135, medium_830_modified_for_dsm_18203, medium_830_modified_for_dsm_18221, medium_830_modified_for_dsm_18603, medium_830_modified_for_dsm_18704, medium_830_modified_for_dsm_18758, medium_830_modified_for_dsm_19096, medium_830_modified_for_dsm_19249, medium_830_modified_for_dsm_19250, medium_830_modified_for_dsm_19309, medium_830_modified_for_dsm_19717, medium_830_modified_for_dsm_19724, medium_830_modified_for_dsm_21036, medium_830_modified_for_dsm_21240, medium_830_modified_for_dsm_21316, medium_830_modified_for_dsm_21346, medium_830_modified_for_dsm_21362, medium_830_modified_for_dsm_21490, medium_830_modified_for_dsm_21568, medium_830_modified_for_dsm_21789, medium_830_modified_for_dsm_21942, medium_830_modified_for_dsm_21994, medium_830_modified_for_dsm_22342, medium_830_modified_for_dsm_22349, medium_830_modified_for_dsm_22405, medium_830_modified_for_dsm_22464, medium_830_modified_for_dsm_22489, medium_830_modified_for_dsm_22489_replace_glucose_with_fructose, medium_830_modified_for_dsm_22869, medium_830_modified_for_dsm_23119, medium_830_modified_for_dsm_23228, medium_830_modified_for_dsm_23491, medium_830_modified_for_dsm_23886, medium_830_modified_for_dsm_23906, medium_830_modified_for_dsm_24448, medium_830_modified_for_dsm_25262, medium_830a_modified_for_dsm_15637, medium_830c_modified_for_dsm_13663, medium_830c_modified_for_dsm_25221, medium_r2a_for_denitrifying_bacteria
2026-08-06T03:05:00.480797+00:00 merge_recipes.py MERGED_RECIPES merged 54 source recipe(s) on fingerprint c0a613e83d57b7c9fa8f6e07ffa6d684870b80cbfdb04c57b4f2bbb3803516da