medium_r2a_for_denitrifying_bacteria

CultureMech:001986 bacterial COMPLEX LIQUID
Original name: MEDIUM R2A FOR DENITRIFYING BACTERIA

Overview

External ontology
mediadive.medium:830b (source link unavailable) — MEDIUM R2A FOR DENITRIFYING BACTERIA
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830b.pdf

Composition

flowchart LR
    CultureMech_001986["medium_r2a_for_denitrifying_bacteria"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001986 --> ing0_Yeast_extract
    ing1_Proteose_peptone["Proteose peptone"]
    CultureMech_001986 --> ing1_Proteose_peptone
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_001986 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_001986 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_001986 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_001986 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001986 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001986 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_KNO3["KNO3"]
    CultureMech_001986 --> ing8_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing8_KNO3 -.-> CHEBI_63043
    ing9_HCl["HCl"]
    CultureMech_001986 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001986 --> ing10_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing11_ZnCl2["ZnCl2"]
    CultureMech_001986 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001986 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_001986 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001986 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001986 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001986 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001986 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.5 G_PER_L
2 Proteose peptone 0.5 G_PER_L
3 Casamino acids 0.5 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
5 Starch 0.5 G_PER_L CHEBI:28017
starch
6 Na-pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 KNO3
K.NO3 101.102 g/mol
0.5 G_PER_L CHEBI:63043
potassium nitrate
10 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
11 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Prepare liquid medium under anaerobic conditions (N2).
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.426268Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 830b
2026-01-28T19:48:38.898200Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:09.123020Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001986
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.641968+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM R2A FOR DENITRIFYING BACTERIA" to "medium_r2a_for_denitrifying_bacteria" for consistent matching
2026-06-05T07:41:30.233399+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:47.572923+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:37:35.436878+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:06.490146+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.