Overview
- External ontology
- komodo.medium:911 (source link unavailable) — MIDDLEBROCK 7H11 AGAR
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_911_modified_for_dsm_44536', 'source': 'KOMODO', 'source_id': 'komodo.medium:911_44536', 'original_category': 'bacterial'}
- Notes
- Source: KOMODO ModelSEED | ID: 911 | DSMZ Medium: 911 (mediadive.medium:911) | Aerobic: No Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_006786["middlebrock_7h11_agar"]
ing0_Glycerol["Glycerol"]
CultureMech_006786 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_006786 --> ing1_Pancreatic_digest_of_casein
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_006786 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_KH2PO4["KH2PO4"]
CultureMech_006786 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Na2HPO4["Na2HPO4"]
CultureMech_006786 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_Sodium_citrate["Sodium citrate"]
CultureMech_006786 --> ing5_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Sodium_citrate -.-> CHEBI_53258
ing6_MgSO4["MgSO4"]
CultureMech_006786 --> ing6_MgSO4
CHEBI_32599["CHEBI:32599"]
ing6_MgSO4 -.-> CHEBI_32599
ing7_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_006786 --> ing7_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing7_L_Glutamic_acid -.-> CHEBI_16015
ing8_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_006786 --> ing8_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing8_Ferric_ammonium_citrate -.-> CHEBI_31604
ing9_Pyridoxine["Pyridoxine"]
CultureMech_006786 --> ing9_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing9_Pyridoxine -.-> CHEBI_16709
ing10_Biotin["Biotin"]
CultureMech_006786 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Malachite_green["Malachite green"]
CultureMech_006786 --> ing11_Malachite_green
CHEBI_72449["CHEBI:72449"]
ing11_Malachite_green -.-> CHEBI_72449
ing12_Agar["Agar"]
CultureMech_006786 --> ing12_Agar
CHEBI_2509["CHEBI:2509"]
ing12_Agar -.-> CHEBI_2509
ing13_Oleic_acid["Oleic acid"]
CultureMech_006786 --> ing13_Oleic_acid
CHEBI_16196["CHEBI:16196"]
ing13_Oleic_acid -.-> CHEBI_16196
ing14_Tryptone["Tryptone"]
CultureMech_006786 --> ing14_Tryptone
ing15_Yeast_extract["Yeast extract"]
CultureMech_006786 --> ing15_Yeast_extract
ing16_Sodium_chloride["Sodium chloride"]
CultureMech_006786 --> ing16_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing16_Sodium_chloride -.-> CHEBI_26710
ing17_Dextrose["Dextrose"]
CultureMech_006786 --> ing17_Dextrose
CHEBI_17634["CHEBI:17634"]
ing17_Dextrose -.-> CHEBI_17634
ing18_Catalase["Catalase"]
CultureMech_006786 --> ing18_Catalase
ing19_NaCl["NaCl"]
CultureMech_006786 --> ing19_NaCl
ing19_NaCl -.-> CHEBI_26710
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
5 G_PER_L |
CHEBI:17754
glycerol
|
|
| 2 | Pancreatic digest of casein | 1.11111 G_PER_L | ||
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.555556 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.66667 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
1.66667 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 6 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.444444 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.0555556 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
0.555556 G_PER_L |
CHEBI:16015
L-glutamic acid
|
|
| 9 | Ferric ammonium citrate | 0.0444444 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 10 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
0.00111111 G_PER_L |
CHEBI:16709
pyridoxine
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.000555556 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Malachite green
C23H25N2.Cl 364.92 g/mol
|
0.00111111 G_PER_L |
CHEBI:72449
malachite green
|
|
| 13 | Agar | 16.6667 G_PER_L |
CHEBI:2509
agar
|
|
| 14 |
Oleic acid
C18H34O2 282.468 g/mol
|
0.5 G_PER_L |
CHEBI:16196
oleic acid
|
|
| 15 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 16 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 17 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 18 |
Dextrose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 19 | Catalase | 0.04 G_PER_L | ||
| 20 |
NaCl
Cl.Na 58.443 g/mol
|
8.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_911_MIDDLEBROCK_7H11_AGAR (CultureMech:006786)
- medium_911_modified_for_dsm_44536 (CultureMech:006785)
- middlebrock_7h11_agar (CultureMech:002079)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 911 |
| 2026-02-03T11:19:11.589262 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 911 |
| 2026-03-13T06:19:43.905891Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006786 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:01.745956+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MIDDLEBROCK 7H11 AGAR" to "middlebrock_7h11_agar" for consistent matching |
| 2026-06-05T07:40:27.724941+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.326310+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:01.795871+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_911_MIDDLEBROCK_7H11_AGAR, medium_911_modified_for_dsm_44536, middlebrock_7h11_agar |
| 2026-08-06T03:05:01.795927+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 1b41b940df4b5db41d26a1005631a9bd05e4614614821f8329c662d154dc2b66 |