Overview
- External ontology
- mediadive.medium:911 (source link unavailable) — MIDDLEBROCK 7H11 AGAR
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.4, 'max': 6.8}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium911.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Final pH at 25 C is 6.6 +/- 0.2; preserve the 6.4-6.8 range without inventing a heating or titration step. Source-specific ingredient identities, quantity bases and preparation routes supersede the copied flattened amounts. Historical events are preserved. Chemical groundings are not comprehensively recertified; no taxa-media association is added.
Composition
flowchart LR
CultureMech_002079["middlebrock_7h11_agar"]
ing0_Glycerol["Glycerol"]
CultureMech_002079 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_Distilled_water["Distilled water"]
CultureMech_002079 --> ing1_Distilled_water
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
0.005 L |
CHEBI:17754
glycerol
|
Source batch input: 5 ml = 0.005 L; not a final-volume marker or a mass concentration. |
| 2 | Distilled water | 0.9 L | Source batch input: 900 ml = 0.9 L; not a final-volume marker or a mass concentration. |
Preparation
- Sterilize for 10 min by 121°C cool to 50 - 55°C. Add 100 ml Bacto Middelbrook OADC enrichment Difco (see below) under aseptic conditions and mix thoroughly.
Solutions
Bacto Middelbrook 7H11 agar (Difco 283810)
| Ingredient | Concentration | Notes |
|---|---|---|
| Pancreatic digest of casein | Source batch amount: 1 g. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=1.11111 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| (NH4)2SO4 | Source batch amount: 0.5 g. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.555556 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| KH2PO4 | Source batch amount: 1.5 g. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=1.66667 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Na2HPO4 | Source batch amount: 1.5 g. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=1.66667 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Sodium citrate | Source batch amount: 0.4 g. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.444444 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| MgSO4 | Source batch amount: 50 mg. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.0555556 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| L-Glutamic acid | Source batch amount: 0.5 g. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.555556 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Ferric ammonium citrate | Source batch amount: 40 mg. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.0444444 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Pyridoxine | Source batch amount: 1 mg. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.00111111 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Biotin | Source batch amount: 0.5 mg. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.000555556 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Malachite green | Source batch amount: 1 mg. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=0.00111111 has its own normalization context and is not adopted as a finished-medium rate. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Agar | Source batch amount: 15 g. Retain this mass in notes; no independently verified final-volume denominator supports a finished-medium g/L conversion. The source API g_l=16.6667 has its own normalization context and is not adopted as a finished-medium rate. Source material qualifier: Bacto . This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. | |
| Distilled water | 0.9 L | Source batch input: 900 ml = 0.9 L; not a final-volume marker or a mass concentration. This is detail of the commercial-base formula, not an additional simultaneous ingredient or water addition to the receiving medium. |
Commercial-base batch input: 21 g. API g_l=11.0236 is not a verified final concentration. Nested composition is source formula detail, not extra simultaneous ingredients; its water is not another receiving-medium addition.
Bacto Middelbrook OADC enrichment (Difco 212240):
Dose: 0.1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Oleic acid | 0.5 G_PER_L | OADC stock input: 50 mg; API stock g_l=0.5 in its 100 ml encoding. This is a provider-reported stock rate, not a finished-medium concentration or independently measured final stock volume. |
| Bovine albumin | 50 G_PER_L | OADC stock input: 5 g; API stock g_l=50 in its 100 ml encoding. This is a provider-reported stock rate, not a finished-medium concentration or independently measured final stock volume. Source material qualifier: albumin fraction V. |
| Dextrose | 20 G_PER_L | OADC stock input: 2 g; API stock g_l=20 in its 100 ml encoding. This is a provider-reported stock rate, not a finished-medium concentration or independently measured final stock volume. |
| Catalase | 0.04 G_PER_L | OADC stock input: 4 mg; API stock g_l=0.04 in its 100 ml encoding. This is a provider-reported stock rate, not a finished-medium concentration or independently measured final stock volume. Source material qualifier: beef. |
| NaCl | 8.5 G_PER_L | OADC stock input: 850 mg; API stock g_l=8.5 in its 100 ml encoding. This is a provider-reported stock rate, not a finished-medium concentration or independently measured final stock volume. |
| Distilled water | 0.1 L | Source batch input: 100 ml = 0.1 L; not a final-volume marker or a mass concentration. |
100 ml = 0.1 L OADC addition after sterilization and cooling, separate from its stock composition. Do not autoclave this later addition or infer a finished-medium denominator.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:12.721777Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 911 |
| 2026-03-13T06:19:10.091762Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002079 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.721548+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MIDDLEBROCK 7H11 AGAR" to "middlebrock_7h11_agar" for consistent matching |
| 2026-05-20T06:01:57.305526+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.4-6.8' -> {'min': 6.4, 'max': 6.8} |
| 2026-06-05T07:41:33.893581+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.831566+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T01:29:53.718683+00:00 | codex | RESTORED_LINKED_MIDDLEBROOK_FORMULATIONS_AND_VARIANT_SCOPES_WITH_EVIDENCE | Restore source-specific Middlebrook ingredients, distinguish commercial/alternative/base/OADC scopes, retain uncertainty and repair reciprocal family links without changing permanent IDs. |