Overview
- External ontology
- komodo.medium:921 (source link unavailable) — METHYLOSARCINA QUISQUILLARUM AND M. FIBRATA MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_921_modified_for_dsm_23269', 'source': 'KOMODO', 'source_id': 'komodo.medium:921_23269', 'original_category': 'bacterial'}
- Notes
- Source: KOMODO ModelSEED | ID: 921 | DSMZ Medium: 921 (mediadive.medium:921) | Aerobic: No
Composition
flowchart LR
CultureMech_006827["methylosarcina_quisquillarum_and_m_fibrata_medium"]
ing0_Agar["Agar"]
CultureMech_006827 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1_Na2_EDTA["Na2-EDTA"]
CultureMech_006827 --> ing1_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing1_Na2_EDTA -.-> CHEBI_64734
ing2_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006827 --> ing2_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing2_CuSO4_x_5_H2O -.-> CHEBI_31440
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006827 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006827 --> ing4_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing4_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing5_H3BO3["H3BO3"]
CultureMech_006827 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006827 --> ing6_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006827 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006827 --> ing8_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
ing9_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_006827 --> ing9_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing9_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing10_KH2PO4["KH2PO4"]
CultureMech_006827 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_KNO3["KNO3"]
CultureMech_006827 --> ing11_KNO3
CHEBI_63043["CHEBI:63043"]
ing11_KNO3 -.-> CHEBI_63043
ing12_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
CultureMech_006827 --> ing12_MgSO4_x_6_H2O
CHEBI_32599["CHEBI:32599"]
ing12_MgSO4_x_6_H2O -.-> CHEBI_32599
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006827 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_006827 --> ing14_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing14_Fe_III__EDTA -.-> CHEBI_30729
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006827 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 2 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 3 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.4 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
0.015 G_PER_L |
CHEBI:33118
boric acid
|
|
| 7 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.05 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.02 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 10 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
71.6 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
26 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
KNO3
K.NO3 101.102 g/mol
|
10 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 13 |
MgSO4 x 6 H2O
Mg.O4S 120.368 g/mol
|
10 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 15 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
3.8 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.26 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_921_METHYLOSARCINA_QUISQUILLARUM_AND_M._FIBRATA_MEDIUM (CultureMech:006827)
- medium_921_modified_for_dsm_23269 (CultureMech:006826)
- methylosarcina_quisquillarum_and_m_fibrata_medium (CultureMech:002090)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 921 |
| 2026-02-03T11:19:14.881162 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 921 |
| 2026-03-13T06:19:44.109975Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006827 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:01.984895+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOSARCINA QUISQUILLARUM AND M. FIBRATA MEDIUM" to "methylosarcina_quisquillarum_and_m_fibrata_medium" for consistent matching |
| 2026-06-05T07:40:27.793987+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.378284+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:46.349554+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:01.922098+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_921_METHYLOSARCINA_QUISQUILLARUM_AND_M._FIBRATA_MEDIUM, medium_921_modified_for_dsm_23269, methylosarcina_quisquillarum_and_m_fibrata_medium |
| 2026-08-06T03:05:01.922146+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 8841902ee930d9032625aff4f342e52b845e8c3fee323c55069e55d2b1a876b5 |