methylosarcina_quisquillarum_and_m_fibrata_medium

Overview

External ontology

mediadive.medium:921 (source link unavailable) — METHYLOSARCINA QUISQUILLARUM AND M. FIBRATA MEDIUM

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium921.pdf

Composition

flowchart LR
    CultureMech_002090["methylosarcina_quisquillarum_and_m_fibrata_medium"]
    ing0_Agar["Agar"]
    CultureMech_002090 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_Na2_EDTA["Na2-EDTA"]
    CultureMech_002090 --> ing1_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing1_Na2_EDTA -.-> CHEBI_64734
    ing2_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002090 --> ing2_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing2_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002090 --> ing3_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing4_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002090 --> ing4_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing4_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing5_H3BO3["H3BO3"]
    CultureMech_002090 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002090 --> ing6_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002090 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002090 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_002090 --> ing9_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing9_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing10_KH2PO4["KH2PO4"]
    CultureMech_002090 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_KNO3["KNO3"]
    CultureMech_002090 --> ing11_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing11_KNO3 -.-> CHEBI_63043
    ing12_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
    CultureMech_002090 --> ing12_MgSO4_x_6_H2O
    CHEBI_32599["CHEBI:32599"]
    ing12_MgSO4_x_6_H2O -.-> CHEBI_32599
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002090 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_002090 --> ing14_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing14_Fe_III__EDTA -.-> CHEBI_30729
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002090 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

Preparation

1. Combine Solutions 1-3 with Trace Elements and distilled water. Then dispense into growth vessels and autoclave under 50% methane in air (or strain specific other ratio) atmosphere (if not using agar).
2. Autoclave at 121 °C for 15 min.
3. Autoclave at 121 °C for 15 min.
4. Add separately autoclaved phosphate buffer (10 ml/L) to autoclaved and cooled medium. If phosphate buffer is added to warm medium, it will precipitate.
5. Liquid cultures should be grown with shaking.
6. May be stored at 4°C in the dark
7. Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre.
8. Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre

Target organisms

No target organisms are recorded.

Provenance