marinitoga_piezophila_medium

CultureMech:006880 bacterial COMPLEX LIQUID
Original name: MARINITOGA PIEZOPHILA medium

Overview

External ontology
komodo.medium:945 (source link unavailable) — MARINITOGA PIEZOPHILA medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'for_dsm_14283', 'source': 'KOMODO', 'source_id': 'komodo.medium:945.1', 'original_category': 'bacterial'}
pH
6.0
Notes
pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 945 | DSMZ Medium: 945 (mediadive.medium:945) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_006880["marinitoga_piezophila_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_006880 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006880 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006880 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KCl["KCl"]
    CultureMech_006880 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaCl["NaCl"]
    CultureMech_006880 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na_acetate["Na-acetate"]
    CultureMech_006880 --> ing5_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Na_acetate -.-> CHEBI_32954
    ing6_MES["MES"]
    CultureMech_006880 --> ing6_MES
    CHEBI_39010["CHEBI:39010"]
    ing6_MES -.-> CHEBI_39010
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_006880 --> ing7_Yeast_extract
    ing8_Trypticase_peptone["Trypticase peptone"]
    CultureMech_006880 --> ing8_Trypticase_peptone
    ing9_K2HPO4["K2HPO4"]
    CultureMech_006880 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_KH2PO4["KH2PO4"]
    CultureMech_006880 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_Sodium_resazurin["Sodium resazurin"]
    CultureMech_006880 --> ing11_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Sodium_resazurin -.-> CHEBI_8806
    ing12_Sulfur["Sulfur"]
    CultureMech_006880 --> ing12_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing12_Sulfur -.-> CHEBI_26833
    ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_006880 --> ing13_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_006880 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_NaOH["NaOH"]
    CultureMech_006880 --> ing15_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing15_NaOH -.-> CHEBI_32145

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
6 Na-acetate
C2H3O2.Na 82.034 g/mol
0.83 G_PER_L CHEBI:32954
sodium acetate
7 MES 1.95 G_PER_L CHEBI:39010
MES
8 Yeast extract 5 G_PER_L
9 Trypticase peptone 5 G_PER_L
10 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
11 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
12 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
13 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom
14 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.3 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
15 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.3 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
16 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 pH adjustment (extracted from notes)

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 945
2026-02-03T11:19:04.406543 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 945
2026-03-13T03:09:03.573939Z data-quality-cleanup-v1.0 Data quality fixes Added pH buffer: NaOH
2026-03-13T06:19:44.591970Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006880
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:02.538718+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MARINITOGA PIEZOPHILA medium" to "marinitoga_piezophila_medium" for consistent matching
2026-05-16T09:03:05.630617+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:28.074718+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.490956+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:02.597075+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: KOMODO_945_MARINITOGA_PIEZOPHILA_medium, for_dsm_14283
2026-08-06T03:05:02.597119+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint a3225c4bf4d3cb60b87a8015cf383058e3195b148a489e1ec73903653b4fc62f