Overview
- External ontology
- komodo.medium:945.1 (source link unavailable) — For DSM 14283
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 945.1 | DSMZ Medium: 945 (mediadive.medium:945) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006878["for_dsm_14283"]
ing0_NH4Cl["NH4Cl"]
CultureMech_006878 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006878 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006878 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_006878 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaCl["NaCl"]
CultureMech_006878 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na_acetate["Na-acetate"]
CultureMech_006878 --> ing5_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Na_acetate -.-> CHEBI_32954
ing6_MES["MES"]
CultureMech_006878 --> ing6_MES
CHEBI_39010["CHEBI:39010"]
ing6_MES -.-> CHEBI_39010
ing7_Yeast_extract["Yeast extract"]
CultureMech_006878 --> ing7_Yeast_extract
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_006878 --> ing8_Trypticase_peptone
ing9_K2HPO4["K2HPO4"]
CultureMech_006878 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_KH2PO4["KH2PO4"]
CultureMech_006878 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_Sodium_resazurin["Sodium resazurin"]
CultureMech_006878 --> ing11_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Sodium_resazurin -.-> CHEBI_8806
ing12_Sulfur["Sulfur"]
CultureMech_006878 --> ing12_Sulfur
CHEBI_26833["CHEBI:26833"]
ing12_Sulfur -.-> CHEBI_26833
ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_006878 --> ing13_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006878 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_NaOH["NaOH"]
CultureMech_006878 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.83 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 | MES | 1.95 G_PER_L |
CHEBI:39010
MES
|
|
| 8 | Yeast extract | 5 G_PER_L | ||
| 9 | Trypticase peptone | 5 G_PER_L | ||
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 14 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 945.1 |
| 2026-02-03T11:19:00.461283 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 945 |
| 2026-03-13T03:08:52.947986Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:44.577717Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006878 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:02.522037+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For DSM 14283" to "for_dsm_14283" for consistent matching |
| 2026-05-16T09:03:32.656727+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:01.415743+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:57.252554+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_945_marinitoga_strain_score10.py | RESOLVED_KOMODO_945_MARINITOGA_STRAIN_TOPOLOGY | KOMODO Medium 945.1 applies MARINITOGA PIEZOPHILA medium to DSM 14283. |