Overview
- External ontology
- MEDIADB:11 (source link unavailable) — Modified m63 medium with ectoine and 2.5 m nacl
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Synonyms
-
- {'name': 'modified_m63_medium_with_ectoine_and_0_6_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:8', 'original_category': 'bacterial'}
- {'name': 'modified_m63_medium_with_ectoine_and_0_75_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:9', 'original_category': 'bacterial'}
- {'name': 'modified_m63_medium_with_ectoine_and_1_5_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:10', 'original_category': 'bacterial'}
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007007["modified_m63_medium_with_ectoine_and_2_5_m_nacl"]
ing0_Ectoine["Ectoine"]
CultureMech_007007 --> ing0_Ectoine
CHEBI_27592["CHEBI:27592"]
ing0_Ectoine -.-> CHEBI_27592
ing1_Potassium_hydroxide["Potassium hydroxide"]
CultureMech_007007 --> ing1_Potassium_hydroxide
CHEBI_32035["CHEBI:32035"]
ing1_Potassium_hydroxide -.-> CHEBI_32035
ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007007 --> ing2_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_007007 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007007 --> ing4_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing4_Magnesium_sulfate -.-> CHEBI_32599
ing5_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007007 --> ing5_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing5_Ferrous_sulfate -.-> CHEBI_75832
ing6_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007007 --> ing6_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing6_Ammonium_sulfate -.-> CHEBI_62946
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Ectoine
C6H10N2O2 142.158 g/mol
|
20.0 MILLIMOLAR |
CHEBI:27592
ectoine
|
|
| 2 |
Potassium hydroxide
HO.K 56.105 g/mol
|
75.0 MILLIMOLAR |
CHEBI:32035
potassium hydroxide
|
|
| 3 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
100.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
|
600.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 5 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.16 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 6 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0018 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 7 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
15.0 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 4 source records.
- modified_m63_medium_with_ectoine_and_0_6_m_nacl (CultureMech:007437)
- modified_m63_medium_with_ectoine_and_0_75_m_nacl (CultureMech:007448)
- modified_m63_medium_with_ectoine_and_1_5_m_nacl (CultureMech:006996)
- modified_m63_medium_with_ectoine_and_2_5_m_nacl (CultureMech:007007)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:42.590507Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 11, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.558255Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007007 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.645774+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified m63 medium with ectoine and 2.5 m nacl" to "modified_m63_medium_with_ectoine_and_2_5_m_nacl" for consistent matching |
| 2026-06-05T07:41:36.934605+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.641524+00:00 | recipe-merger | Merged 4 duplicate recipes into canonical record | Sources: modified_m63_medium_with_ectoine_and_0_6_m_nacl, modified_m63_medium_with_ectoine_and_0_75_m_nacl, modified_m63_medium_with_ectoine_and_1_5_m_nacl, modified_m63_medium_with_ectoine_and_2_5_m_nacl |
| 2026-08-06T03:05:48.641590+00:00 | merge_recipes.py | MERGED_RECIPES | merged 4 source recipe(s) on fingerprint 45d242b52b11a53beaef734ea7584b00bec40f29c890b55b02c3648a3b3ec5dc |