modified_m63_medium_with_ectoine_and_0_75_m_nacl

CultureMech:007448 bacterial DEFINED LIQUID
Original name: Modified m63 medium with ectoine and 0.75 m nacl

Overview

External ontology
MEDIADB:9 (source link unavailable) — Modified m63 medium with ectoine and 0.75 m nacl
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007448["modified_m63_medium_with_ectoine_and_0_75_m_nacl"]
    ing0_Ectoine["Ectoine"]
    CultureMech_007448 --> ing0_Ectoine
    CHEBI_27592["CHEBI:27592"]
    ing0_Ectoine -.-> CHEBI_27592
    ing1_Potassium_hydroxide["Potassium hydroxide"]
    CultureMech_007448 --> ing1_Potassium_hydroxide
    CHEBI_32035["CHEBI:32035"]
    ing1_Potassium_hydroxide -.-> CHEBI_32035
    ing2_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007448 --> ing2_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing2_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007448 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Magnesium_sulfate_heptahydrate["Magnesium sulfate heptahydrate"]
    CultureMech_007448 --> ing4_Magnesium_sulfate_heptahydrate
    CHEBI_31795["CHEBI:31795"]
    ing4_Magnesium_sulfate_heptahydrate -.-> CHEBI_31795
    ing5_Ferrous_sulfate_heptahydrate["Ferrous sulfate heptahydrate"]
    CultureMech_007448 --> ing5_Ferrous_sulfate_heptahydrate
    CHEBI_75836["CHEBI:75836"]
    ing5_Ferrous_sulfate_heptahydrate -.-> CHEBI_75836
    ing6_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007448 --> ing6_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing6_Ammonium_sulfate -.-> CHEBI_62946

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Ectoine
C6H10N2O2 142.158 g/mol
20.0 MILLIMOLAR CHEBI:27592
ectoine
2 Potassium hydroxide
HO.K 56.105 g/mol
75.0 MILLIMOLAR CHEBI:32035
potassium hydroxide
3 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
100.0 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
4 Sodium chloride
Cl.Na 58.443 g/mol
750.0 MILLIMOLAR CHEBI:26710
sodium chloride
5 Magnesium sulfate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
0.16 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
6 Ferrous sulfate heptahydrate
Fe.7H2O.O4S 278.013 g/mol
0.0018 MILLIMOLAR CHEBI:75836
iron(2+) sulfate heptahydrate
7 Ammonium sulfate
2H4N.O4S 132.141 g/mol
15.0 MILLIMOLAR CHEBI:62946
ammonium sulfate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:42.571408Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 9, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:49.116023Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007448
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.505281+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified m63 medium with ectoine and 0.75 m nacl" to "modified_m63_medium_with_ectoine_and_0_75_m_nacl" for consistent matching
2026-06-05T07:41:36.922743+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-09T21:06:17.385933+00:00 codex CURATED_M63_PRIMARY_AMOUNTS_AND_CHEMICAL_FORMS Added primary-source concentration snippets and corrected phosphate/sulfate chemical forms using the explicitly cited M63 definition. Five base-medium amounts and three chemical forms follow the 1982 M63 definition explicitly cited as reference 48 by the 2010 Methods. This cross-reference inference is distinct from the carbon and salt concentrations directly reported in 2010. KOH at 75 mM is a base-recipe input, not a quantified pH-adjustment dose. No fixed titration dose is inferred from the separate 2010 pH 7.2 adjustment. Existing D-glucose stereochemistry and hydroxyectoine positional naming are preserved, not independently certified by the amount evidence. No quantity, preparation, classification, growth association or variant-link changes. These records are not certified as complete primary-source formulations.