Overview
- External ontology
- MEDIADB:140 (source link unavailable) — Mmsuc; jasiecki et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007030["mmsuc_jasiecki_et_al"]
ing0_Thiamine["Thiamine"]
CultureMech_007030 --> ing0_Thiamine
CHEBI_18385["CHEBI:18385"]
ing0_Thiamine -.-> CHEBI_18385
ing1_Maleic_acid["Maleic acid"]
CultureMech_007030 --> ing1_Maleic_acid
CHEBI_18300["CHEBI:18300"]
ing1_Maleic_acid -.-> CHEBI_18300
ing2_Tromethamine["Tromethamine"]
CultureMech_007030 --> ing2_Tromethamine
CHEBI_9754["CHEBI:9754"]
ing2_Tromethamine -.-> CHEBI_9754
ing3_Magnesium_chloride["Magnesium chloride"]
CultureMech_007030 --> ing3_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing3_Magnesium_chloride -.-> CHEBI_86345
ing4_Sodium_sulfate["Sodium sulfate"]
CultureMech_007030 --> ing4_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing4_Sodium_sulfate -.-> CHEBI_32149
ing5_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007030 --> ing5_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_007030 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Potassium_chloride["Potassium chloride"]
CultureMech_007030 --> ing7_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing7_Potassium_chloride -.-> CHEBI_32588
ing8_Ammonium_chloride["Ammonium chloride"]
CultureMech_007030 --> ing8_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing8_Ammonium_chloride -.-> CHEBI_31206
ing9_Sodium_Succinate["Sodium Succinate"]
CultureMech_007030 --> ing9_Sodium_Succinate
CHEBI_63675["CHEBI:63675"]
ing9_Sodium_Succinate -.-> CHEBI_63675
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.037684 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 2 |
Maleic acid
C4H4O4 116.072 g/mol
|
49.9698 MILLIMOLAR |
CHEBI:18300
maleic acid
|
Cross-references: KEGG:male |
| 3 |
Tromethamine
C4H11NO3 121.136 g/mol
|
49.5295 MILLIMOLAR |
CHEBI:9754
tris
|
|
| 4 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
4.91859 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
0.929316 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 6 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
0.898939 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
|
42.7789 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 8 |
Potassium chloride
Cl.K 74.551 g/mol
|
26.8272 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 9 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
18.6947 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 10 |
Sodium Succinate
C4H4O4.2Na 162.052 g/mol
|
37.026 MILLIMOLAR |
CHEBI:63675
sodium succinate (anhydrous)
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mmsuc_jasiecki_et_al (CultureMech:007030)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.849844Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 140, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:38.128744Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.679576Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007030 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.779965+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mmsuc; jasiecki et al" to "mmsuc_jasiecki_et_al" for consistent matching |
| 2026-06-05T07:41:35.782784+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:47.555979+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint af0ad12f7fd658bfac4072a270e08ff857aff55c40fe5792eb5dc5722ffdb8f2 |