mmsuc_jasiecki_et_al

CultureMech:007030 bacterial DEFINED LIQUID
Original name: Mmsuc; jasiecki et al

Overview

External ontology
MEDIADB:140 (source link unavailable) — Mmsuc; jasiecki et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007030["mmsuc_jasiecki_et_al"]
    ing0_Thiamine["Thiamine"]
    CultureMech_007030 --> ing0_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing0_Thiamine -.-> CHEBI_18385
    ing1_Maleic_acid["Maleic acid"]
    CultureMech_007030 --> ing1_Maleic_acid
    CHEBI_18300["CHEBI:18300"]
    ing1_Maleic_acid -.-> CHEBI_18300
    ing2_Tromethamine["Tromethamine"]
    CultureMech_007030 --> ing2_Tromethamine
    CHEBI_9754["CHEBI:9754"]
    ing2_Tromethamine -.-> CHEBI_9754
    ing3_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007030 --> ing3_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing3_Magnesium_chloride -.-> CHEBI_86345
    ing4_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007030 --> ing4_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing4_Sodium_sulfate -.-> CHEBI_32149
    ing5_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007030 --> ing5_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing5_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing6_Sodium_chloride["Sodium chloride"]
    CultureMech_007030 --> ing6_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing6_Sodium_chloride -.-> CHEBI_26710
    ing7_Potassium_chloride["Potassium chloride"]
    CultureMech_007030 --> ing7_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing7_Potassium_chloride -.-> CHEBI_32588
    ing8_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007030 --> ing8_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing8_Ammonium_chloride -.-> CHEBI_31206
    ing9_Sodium_Succinate["Sodium Succinate"]
    CultureMech_007030 --> ing9_Sodium_Succinate
    CHEBI_63675["CHEBI:63675"]
    ing9_Sodium_Succinate -.-> CHEBI_63675

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Thiamine
C12H17N4OS 265.362 g/mol
0.037684 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
2 Maleic acid
C4H4O4 116.072 g/mol
49.9698 MILLIMOLAR CHEBI:18300
maleic acid
 Cross-references: KEGG:male
3 Tromethamine
C4H11NO3 121.136 g/mol
49.5295 MILLIMOLAR CHEBI:9754
tris
4 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
4.91859 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
5 Sodium sulfate
2Na.O4S 142.043 g/mol
0.929316 MILLIMOLAR CHEBI:32149
sodium sulfate
6 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
0.898939 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
7 Sodium chloride
Cl.Na 58.443 g/mol
42.7789 MILLIMOLAR CHEBI:26710
sodium chloride
8 Potassium chloride
Cl.K 74.551 g/mol
26.8272 MILLIMOLAR CHEBI:32588
potassium chloride
9 Ammonium chloride
Cl.H4N 53.492 g/mol
18.6947 MILLIMOLAR CHEBI:31206
ammonium chloride
10 Sodium Succinate
C4H4O4.2Na 162.052 g/mol
37.026 MILLIMOLAR CHEBI:63675
sodium succinate (anhydrous)

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.849844Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 140, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:38.128744Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.679576Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007030
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.779965+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Mmsuc; jasiecki et al" to "mmsuc_jasiecki_et_al" for consistent matching
2026-06-05T07:41:35.782784+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.