Overview
- External ontology
- MEDIADB:143 (source link unavailable) — A+B Salts Medium with Uracil; Jensen et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007033["a_b_salts_medium_with_uracil_jensen_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007033 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Uracil["Uracil"]
CultureMech_007033 --> ing1_Uracil
CHEBI_17568["CHEBI:17568"]
ing1_Uracil -.-> CHEBI_17568
ing2_Thiamine["Thiamine"]
CultureMech_007033 --> ing2_Thiamine
CHEBI_18385["CHEBI:18385"]
ing2_Thiamine -.-> CHEBI_18385
ing3_Magnesium_chloride["Magnesium chloride"]
CultureMech_007033 --> ing3_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing3_Magnesium_chloride -.-> CHEBI_86345
ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007033 --> ing4_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing5_Sodium_sulfate["Sodium sulfate"]
CultureMech_007033 --> ing5_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing5_Sodium_sulfate -.-> CHEBI_32149
ing6_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007033 --> ing6_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing6_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_007033 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007033 --> ing8_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing9_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007033 --> ing9_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing9_Ammonium_sulfate -.-> CHEBI_62946
ing10__Iron_III["'Iron(III"]
CultureMech_007033 --> ing10__Iron_III
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
11.1012 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Uracil
C4H4N2O2 112.088 g/mol
|
0.0178433 MILLIMOLAR |
CHEBI:17568
uracil
|
Cross-references: KEGG:ura |
| 3 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.0037684 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 4 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
2.10059 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.090102 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 6 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
0.0774429 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 7 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
42.2654 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
|
51.3347 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 9 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
22.0449 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
15.1355 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 11 | 'Iron(III | 0.00184979 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- a_b_salts_medium_with_uracil_jensen_et_al (CultureMech:007033)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.877540Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 143, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:36.497570Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.696973Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007033 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.799311+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A+B Salts Medium with Uracil; Jensen et al" to "a_b_salts_medium_with_uracil_jensen_et_al" for consistent matching |
| 2026-06-05T07:40:46.470876+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:21.160471+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3abf2de051f1acb96c4f0007ee26c788c32d13135f4c1573f85d2af1c65a55ce |