a_b_salts_medium_with_uracil_jensen_et_al

CultureMech:007033 bacterial DEFINED LIQUID
Original name: A+B Salts Medium with Uracil; Jensen et al

Overview

External ontology
MEDIADB:143 (source link unavailable) — A+B Salts Medium with Uracil; Jensen et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007033["a_b_salts_medium_with_uracil_jensen_et_al"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007033 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Uracil["Uracil"]
    CultureMech_007033 --> ing1_Uracil
    CHEBI_17568["CHEBI:17568"]
    ing1_Uracil -.-> CHEBI_17568
    ing2_Thiamine["Thiamine"]
    CultureMech_007033 --> ing2_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing2_Thiamine -.-> CHEBI_18385
    ing3_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007033 --> ing3_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing3_Magnesium_chloride -.-> CHEBI_86345
    ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007033 --> ing4_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing5_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007033 --> ing5_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing5_Sodium_sulfate -.-> CHEBI_32149
    ing6_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007033 --> ing6_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing6_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing7_Sodium_chloride["Sodium chloride"]
    CultureMech_007033 --> ing7_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing7_Sodium_chloride -.-> CHEBI_26710
    ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007033 --> ing8_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing9_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007033 --> ing9_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing9_Ammonium_sulfate -.-> CHEBI_62946
    ing10_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007033 --> ing10_Iron_III__chloride

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
11.1012 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Uracil
C4H4N2O2 112.088 g/mol
0.0178433 MILLIMOLAR CHEBI:17568
uracil
 Cross-references: KEGG:ura
3 Thiamine
C12H17N4OS 265.362 g/mol
0.0037684 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
4 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
2.10059 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
5 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.090102 MILLIMOLAR CHEBI:3312
calcium dichloride
6 Sodium sulfate
2Na.O4S 142.043 g/mol
0.0774429 MILLIMOLAR CHEBI:32149
sodium sulfate
7 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
42.2654 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
8 Sodium chloride
Cl.Na 58.443 g/mol
51.3347 MILLIMOLAR CHEBI:26710
sodium chloride
9 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
22.0449 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
10 Ammonium sulfate
2H4N.O4S 132.141 g/mol
15.1355 MILLIMOLAR CHEBI:62946
ammonium sulfate
11 Iron(III) chloride 0.00184979 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.877540Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 143, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:36.497570Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.696973Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007033
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.799311+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "A+B Salts Medium with Uracil; Jensen et al" to "a_b_salts_medium_with_uracil_jensen_et_al" for consistent matching
2026-06-05T07:40:46.470876+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:06:06.870125+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:143's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.