Overview
- External ontology
- MEDIADB:175 (source link unavailable) — Gonzales et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007066["gonzales_et_al"]
ing0_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007066 --> ing0_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing0_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing1_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007066 --> ing1_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing1_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing2_Sodium_chloride["Sodium chloride"]
CultureMech_007066 --> ing2_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing2_Sodium_chloride -.-> CHEBI_26710
ing3_Potassium_chloride["Potassium chloride"]
CultureMech_007066 --> ing3_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing3_Potassium_chloride -.-> CHEBI_32588
ing4_Sodium_acetate["Sodium acetate"]
CultureMech_007066 --> ing4_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Sodium_acetate -.-> CHEBI_32954
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007066 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Ammonium_chloride["Ammonium chloride"]
CultureMech_007066 --> ing6_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing6_Ammonium_chloride -.-> CHEBI_31206
ing7_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007066 --> ing7_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing7_Ferrous_sulfate -.-> CHEBI_75832
ing8_Zinc_sulfate["Zinc sulfate"]
CultureMech_007066 --> ing8_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing8_Zinc_sulfate -.-> CHEBI_35176
ing9_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
CultureMech_007066 --> ing9_Sodium_dihydrogen_phosphate
CHEBI_37585["CHEBI:37585"]
ing9_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
ing10_Manganese_chloride["Manganese chloride"]
CultureMech_007066 --> ing10_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing10_Manganese_chloride -.-> CHEBI_63041
ing11_Cupric_sulfate["Cupric sulfate"]
CultureMech_007066 --> ing11_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing11_Cupric_sulfate -.-> CHEBI_23414
ing12_Sodium_carbonate["Sodium carbonate"]
CultureMech_007066 --> ing12_Sodium_carbonate
CHEBI_29377["CHEBI:29377"]
ing12_Sodium_carbonate -.-> CHEBI_29377
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.003 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 2 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
2.0 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
Sodium chloride
Cl.Na 58.443 g/mol
|
3400.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 4 |
Potassium chloride
Cl.K 74.551 g/mol
|
27.0 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 5 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
25.0 MILLIMOLAR |
CHEBI:32954
sodium acetate
|
|
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
1.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 7 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
12.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 8 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.005 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 9 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.003 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 10 |
Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
|
2.0 MILLIMOLAR |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 11 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.004 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 12 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.004 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 13 |
Sodium carbonate
CO3.2Na 105.988 g/mol
|
175.0 MILLIMOLAR |
CHEBI:29377
sodium carbonate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- gonzales_et_al (CultureMech:007066)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.150242Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 175, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:15.638622Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.876918Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007066 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.993124+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Gonzales et al" to "gonzales_et_al" for consistent matching |
| 2026-06-05T07:41:06.602088+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-09T01:42:20.649822+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:36.396839+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 03b92a3a15464d76d8ed18a97f7eeeba4d73dff0ff1aedef7cab31abcae59f2c |