gonzales_et_al

CultureMech:007066 bacterial DEFINED LIQUID
Original name: Gonzales et al

Overview

External ontology
MEDIADB:175 (source link unavailable) — Gonzales et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007066["gonzales_et_al"]
    ing0_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007066 --> ing0_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing0_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing1_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007066 --> ing1_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing1_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing2_Sodium_chloride["Sodium chloride"]
    CultureMech_007066 --> ing2_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_chloride -.-> CHEBI_26710
    ing3_Potassium_chloride["Potassium chloride"]
    CultureMech_007066 --> ing3_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing3_Potassium_chloride -.-> CHEBI_32588
    ing4_Sodium_acetate["Sodium acetate"]
    CultureMech_007066 --> ing4_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate -.-> CHEBI_32954
    ing5_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007066 --> ing5_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing5_Magnesium_sulfate -.-> CHEBI_32599
    ing6_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007066 --> ing6_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing6_Ammonium_chloride -.-> CHEBI_31206
    ing7_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007066 --> ing7_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing7_Ferrous_sulfate -.-> CHEBI_75832
    ing8_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007066 --> ing8_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing8_Zinc_sulfate -.-> CHEBI_35176
    ing9_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
    CultureMech_007066 --> ing9_Sodium_dihydrogen_phosphate
    CHEBI_37585["CHEBI:37585"]
    ing9_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
    ing10_Manganese_chloride["Manganese chloride"]
    CultureMech_007066 --> ing10_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing10_Manganese_chloride -.-> CHEBI_63041
    ing11_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007066 --> ing11_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing11_Cupric_sulfate -.-> CHEBI_23414
    ing12_Sodium_carbonate["Sodium carbonate"]
    CultureMech_007066 --> ing12_Sodium_carbonate
    CHEBI_29377["CHEBI:29377"]
    ing12_Sodium_carbonate -.-> CHEBI_29377

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.003 MILLIMOLAR CHEBI:3312
calcium dichloride
2 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
2.0 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
3 Sodium chloride
Cl.Na 58.443 g/mol
3400.0 MILLIMOLAR CHEBI:26710
sodium chloride
4 Potassium chloride
Cl.K 74.551 g/mol
27.0 MILLIMOLAR CHEBI:32588
potassium chloride
5 Sodium acetate
C2H3O2.Na 82.034 g/mol
25.0 MILLIMOLAR CHEBI:32954
sodium acetate
6 Magnesium sulfate
Mg.O4S 120.368 g/mol
1.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
7 Ammonium chloride
Cl.H4N 53.492 g/mol
12.0 MILLIMOLAR CHEBI:31206
ammonium chloride
8 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.005 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
9 Zinc sulfate
O4S.Zn 161.453 g/mol
0.003 MILLIMOLAR CHEBI:35176
zinc sulfate
10 Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
2.0 MILLIMOLAR CHEBI:37585
sodium dihydrogenphosphate
11 Manganese chloride
Cl2Mn 125.844 g/mol
0.004 MILLIMOLAR CHEBI:63041
manganese(II) chloride
12 Cupric sulfate
Cu.O4S 159.609 g/mol
0.004 MILLIMOLAR CHEBI:23414
copper(II) sulfate
13 Sodium carbonate
CO3.2Na 105.988 g/mol
175.0 MILLIMOLAR CHEBI:29377
sodium carbonate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.150242Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 175, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:15.638622Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.876918Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007066
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.993124+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Gonzales et al" to "gonzales_et_al" for consistent matching
2026-06-05T07:41:06.602088+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-09T01:42:20.649822+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).