Overview
- External ontology
- MEDIADB:226 (source link unavailable) — Inorganic medium
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Synonyms
-
- {'name': 'fex5_medium', 'source': 'unknown', 'source_id': 'MEDIADB:227', 'original_category': 'bacterial'}
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007121["inorganic_medium"]
ing0_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007121 --> ing0_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing0_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing1_Boric_acid["Boric acid"]
CultureMech_007121 --> ing1_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing1_Boric_acid -.-> CHEBI_33118
ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007121 --> ing2_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_007121 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007121 --> ing4_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007121 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Manganese_sulfate["Manganese sulfate"]
CultureMech_007121 --> ing6_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing6_Manganese_sulfate -.-> CHEBI_86364
ing7_Ammonium_chloride["Ammonium chloride"]
CultureMech_007121 --> ing7_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing7_Ammonium_chloride -.-> CHEBI_31206
ing8_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007121 --> ing8_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing8_Ferrous_sulfate -.-> CHEBI_75832
ing9_Zinc_sulfate["Zinc sulfate"]
CultureMech_007121 --> ing9_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing9_Zinc_sulfate -.-> CHEBI_35176
ing10_Cupric_chloride["Cupric chloride"]
CultureMech_007121 --> ing10_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing10_Cupric_chloride -.-> CHEBI_49553
ing11_Cupric_sulfate["Cupric sulfate"]
CultureMech_007121 --> ing11_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing11_Cupric_sulfate -.-> CHEBI_23414
ing12_Molybdenum_trioxide["Molybdenum trioxide"]
CultureMech_007121 --> ing12_Molybdenum_trioxide
CHEBI_30627["CHEBI:30627"]
ing12_Molybdenum_trioxide -.-> CHEBI_30627
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.27 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 2 |
Boric acid
H3BO3 61.833 g/mol
|
3.2e-05 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 3 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
11.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
|
4.3 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 5 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
7.3 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 7 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
8.3e-06 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 8 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
56.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.25 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 10 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
5.7e-06 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 11 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
8.4e-06 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 12 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
4e-06 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 13 |
Molybdenum trioxide
MoO3 143.937 g/mol
|
1.4e-05 MILLIMOLAR |
CHEBI:30627
molybdenum trioxide
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- fex5_medium (CultureMech:007122)
- inorganic_medium (CultureMech:007121)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.677526Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 226, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.311297Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007121 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.460973+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Inorganic medium" to "inorganic_medium" for consistent matching |
| 2026-06-05T07:41:09.352086+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:34.373992+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: fex5_medium, inorganic_medium |
| 2026-08-06T03:05:34.374031+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 648c6d7225801c0199b353b050958730707a4856451f06a29f875ed945f9dbd7 |