fex5_medium

CultureMech:007122 bacterial DEFINED LIQUID
Original name: Fex5 medium

Overview

External ontology
MEDIADB:227 (source link unavailable) — Fex5 medium
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007122["fex5_medium"]
    ing0_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007122 --> ing0_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing0_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing1_Boric_acid["Boric acid"]
    CultureMech_007122 --> ing1_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing1_Boric_acid -.-> CHEBI_33118
    ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007122 --> ing2_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007122 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007122 --> ing4_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing5_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007122 --> ing5_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing5_Magnesium_sulfate -.-> CHEBI_32599
    ing6_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007122 --> ing6_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing6_Manganese_sulfate -.-> CHEBI_86364
    ing7_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007122 --> ing7_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing7_Ammonium_chloride -.-> CHEBI_31206
    ing8_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007122 --> ing8_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing8_Ferrous_sulfate -.-> CHEBI_75832
    ing9_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007122 --> ing9_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing9_Zinc_sulfate -.-> CHEBI_35176
    ing10_Cupric_chloride["Cupric chloride"]
    CultureMech_007122 --> ing10_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing10_Cupric_chloride -.-> CHEBI_49553
    ing11_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007122 --> ing11_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing11_Cupric_sulfate -.-> CHEBI_23414
    ing12_Molybdenum_trioxide["Molybdenum trioxide"]
    CultureMech_007122 --> ing12_Molybdenum_trioxide
    CHEBI_30627["CHEBI:30627"]
    ing12_Molybdenum_trioxide -.-> CHEBI_30627

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.27 MILLIMOLAR CHEBI:3312
calcium dichloride
2 Boric acid
H3BO3 61.833 g/mol
3.2e-05 MILLIMOLAR CHEBI:33118
boric acid
3 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
11.0 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
4 Sodium chloride
Cl.Na 58.443 g/mol
4.3 MILLIMOLAR CHEBI:26710
sodium chloride
5 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
7.3 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
6 Magnesium sulfate
Mg.O4S 120.368 g/mol
2.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
7 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
8.3e-06 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
8 Ammonium chloride
Cl.H4N 53.492 g/mol
56.0 MILLIMOLAR CHEBI:31206
ammonium chloride
9 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.25 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
10 Zinc sulfate
O4S.Zn 161.453 g/mol
5.7e-06 MILLIMOLAR CHEBI:35176
zinc sulfate
11 Cupric chloride
Cl2Cu 134.452 g/mol
8.4e-06 MILLIMOLAR CHEBI:49553
copper(II) chloride
12 Cupric sulfate
Cu.O4S 159.609 g/mol
4e-06 MILLIMOLAR CHEBI:23414
copper(II) sulfate
13 Molybdenum trioxide
MoO3 143.937 g/mol
1.4e-05 MILLIMOLAR CHEBI:30627
molybdenum trioxide

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.685696Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 227, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.317341Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007122
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.467659+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Fex5 medium" to "fex5_medium" for consistent matching
2026-06-05T07:41:00.346944+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.